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PSMC6 Protein (AA 1-389) (His tag)

Crystallography grade PSMC6 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
产品编号 ABIN3086581
发货至: 中国
  • 抗原 See all PSMC6 蛋白
    PSMC6 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 6 (PSMC6))
    蛋白类型
    Recombinant
    产品特性
    AA 1-389
    宿主
    • 5
    • 1
    • 1
    资源
    • 2
    • 2
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This PSMC6 protein is labelled with His tag.
    应用范围
    Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
    序列
    MADPRDKALQ DYRKKLLEHK EIDGRLKELR EQLKELTKQY EKSENDLKAL QSVGQIVGEV LKQLTEEKFI VKATNGPRYV VGCRRQLDKS KLKPGTRVAL DMTTLTIMRY LPREVDPLVY NMSHEDPGNV SYSEIGGLSE QIRELREVIE LPLTNPELFQ RVGIIPPKGC LLYGPPGTGK TLLARAVASQ LDCNFLKVVS SSIVDKYIGE SARLIREMFN YARDHQPCII FMDEIDAIGG RRFSEGTSAD REIQRTLMEL LNQMDGFDTL HRVKMIMATN RPDTLDPALL RPGRLDRKIH IDLPNEQARL DILKIHAGPI TKHGEIDYEA IVKLSDGFNG ADLRNVCTEA GMFAIRADHD FVVQEDFMKA VRKVADSKKL ESKLDYKPV
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PSMC6 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product PSMC6 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    PSMC6 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 6 (PSMC6))
    别名
    PSMC6 (PSMC6 产品)
    别名
    DDBDRAFT_0186051 Protein, DDBDRAFT_0232968 Protein, DDB_0186051 Protein, DDB_0232968 Protein, fb20g04 Protein, wu:fb20g04 Protein, wu:fc85c10 Protein, cadp44 Protein, sug2 Protein, PSMC6 Protein, psmc6-a Protein, NV13405 Protein, CADP44 Protein, P44 Protein, SUG2 Protein, p42 Protein, 2300001E01Rik Protein, AI451058 Protein, 26S protease regulatory subunit S10B Protein, 26S proteasome regulatory subunit 10B Protein, proteasome 26S subunit, ATPase 6 Protein, proteasome 26S subunit, ATPase 6 L homeolog Protein, 26S protease regulatory subunit 10B Protein, proteasome 26S subunit, ATPase 6 S homeolog Protein, proteasome (prosome, macropain) 26S subunit, ATPase, 6 Protein, ATEG_02903 Protein, HCAG_04181 Protein, LOC5565051 Protein, EDI_289460 Protein, CpipJ_CPIJ003334 Protein, EHI_194070 Protein, PTRG_02716 Protein, BDBG_01801 Protein, psmC6 Protein, MCYG_07206 Protein, PITG_00229 Protein, VDBG_04729 Protein, MGYG_04704 Protein, TERG_01608 Protein, DICPUDRAFT_97252 Protein, Tsp_02519 Protein, prs10 Protein, LOC100282100 Protein, Psmc6 Protein, psmc6 Protein, psmc6.L Protein, PSMC6 Protein, LOC693577 Protein, psmc6.S Protein, LOC100117671 Protein
    背景
    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
    分子量
    45.1 kDa Including tag.
    UniProt
    P62333
    途径
    Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway
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