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PSMC3 Protein (AA 1-439) (His tag)

Crystallography grade PSMC3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
产品编号 ABIN3086525
发货至: 中国
  • 抗原 See all PSMC3 蛋白
    PSMC3 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3 (PSMC3))
    蛋白类型
    Recombinant
    产品特性
    AA 1-439
    宿主
    • 4
    • 1
    • 1
    • 1
    资源
    • 2
    • 2
    • 1
    • 1
    • 1
    Insect Cells
    标记
    This PSMC3 protein is labelled with His tag.
    应用范围
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    序列
    MNLLPNIESP VTRQEKMATV WDEAEQDGIG EEVLKMSTEE IIQRTRLLDS EIKIMKSEVL RVTHELQAMK DKIKENSEKI KVNKTLPYLV SNVIELLDVD PNDQEEDGAN IDLDSQRKGK CAVIKTSTRQ TYFLPVIGLV DAEKLKPGDL VGVNKDSYLI LETLPTEYDS RVKAMEVDER PTEQYSDIGG LDKQIQELVE AIVLPMNHKE KFENLGIQPP KGVLMYGPPG TGKTLLARAC AAQTKATFLK LAGPQLVQMF IGDGAKLVRD AFALAKEKAP SIIFIDELDA IGTKRFDSEK AGDREVQRTM LELLNQLDGF QPNTQVKVIA ATNRVDILDP ALLRSGRLDR KIEFPMPNEE ARARIMQIHS RKMNVSPDVN YEELARCTDD FNGAQCKAVC VEAGMIALRR GATELTHEDY MEGILEVQAK KKANLQYYA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PSMC3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product PSMC3 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    PSMC3 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3 (PSMC3))
    别名
    PSMC3 (PSMC3 产品)
    别名
    TBP1 Protein, TBP-1 Protein, wu:fb39a02 Protein, zgc:86713 Protein, psmc3 Protein, MGC89608 Protein, PSMC3 Protein, NV15940 Protein, 23.m06430 Protein, DDBDRAFT_0186002 Protein, DDBDRAFT_0232967 Protein, DDB_0186002 Protein, DDB_0232967 Protein, psmc3-b Protein, tbp10 Protein, psmc3-a Protein, proteasome 26S subunit, ATPase 3 Protein, proteasome (prosome, macropain) 26S subunit, ATPase 3 Protein, 26S protease regulatory subunit 6A Protein, 26S protease regulatory subunit 6a Protein, 26S protease regulatory subunit 6A-B Protein, 26S protease regulatory subunit 6A, putative Protein, proteasome 26S subunit, ATPase 3 S homeolog Protein, nth-like DNA glycosylase 1 S homeolog Protein, PSMC3 Protein, Psmc3 Protein, HCAG_00069 Protein, GL50803_4365 Protein, EDI_316920 Protein, CpipJ_CPIJ006888 Protein, CpipJ_CPIJ006889 Protein, CpipJ_CPIJ010864 Protein, prs6a Protein, psmc3 Protein, LOC100115023 Protein, BBOV_IV004580 Protein, CGB_M2140W Protein, psmC3 Protein, LOC100637883 Protein, psmc3.S Protein, nthl1.S Protein
    背景
    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation. {ECO:0000250}.
    分子量
    50.2 kDa Including tag.
    UniProt
    P17980
    途径
    Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway
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