PFKM Protein (AA 2-780) (His tag)
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- 抗原 See all PFKM 蛋白
- PFKM (phosphofructokinase, Muscle (PFKM))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-780
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宿主
- 人
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资源
- Insect Cells
- 标记
- This PFKM protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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THEEHHAAKT LGIGKAIAVL TSGGDAQGMN AAVRAVVRVG IFTGARVFFV HEGYQGLVDG GDHIKEATWE SVSMMLQLGG TVIGSARCKD FREREGRLRA AYNLVKRGIT NLCVIGGDGS LTGADTFRSE WSDLLSDLQK AGKITDEEAT KSSYLNIVGL VGSIDNDFCG TDMTIGTDSA LHRIMEIVDA ITTTAQSHQR TFVLEVMGRH CGYLALVTSL SCGADWVFIP ECPPDDDWEE HLCRRLSETR TRGSRLNIII VAEGAIDKNG KPITSEDIKN LVVKRLGYDT RVTVLGHVQR GGTPSAFDRI LGSRMGVEAV MALLEGTPDT PACVVSLSGN QAVRLPLMEC VQVTKDVTKA MDEKKFDEAL KLRGRSFMNN WEVYKLLAHV RPPVSKSGSH TVAVMNVGAP AAGMNAAVRS TVRIGLIQGN RVLVVHDGFE GLAKGQIEEA GWSYVGGWTG QGGSKLGTKR TLPKKSFEQI SANITKFNIQ GLVIIGGFEA YTGGLELMEG RKQFDELCIP FVVIPATVSN NVPGSDFSVG ADTALNTICT TCDRIKQSAA GTKRRVFIIE TMGGYCGYLA TMAGLAAGAD AAYIFEEPFT IRDLQANVEH LVQKMKTTVK RGLVLRNEKC NENYTTDFIF NLYSEEGKGI FDSRKNVLGH MQQGGSPTPF DRNFATKMGA KAMNWMSGKI KESYRNGRIF ANTPDSGCVL GMRKRALVFQ PVAELKDQTD FEHRIPKEQW WLKLRPILKI LAKYEIDLDT SDHAHLEHIT RKRSGEAAV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human PFKM Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product PFKM 蛋白
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phosphofructokinase, Muscle (PFKM) (AA 1-780) protein (His tag)
PFKM 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % by SDS - PAGE SDS
phosphofructokinase, Muscle (PFKM) (AA 681-780) protein (GST tag)PFKM 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
phosphofructokinase, Muscle (PFKM) protein (GST tag,His tag)PFKM 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
phosphofructokinase, Muscle (PFKM) (AA 2-780) protein (His tag)Crystallography grade PFKM 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
phosphofructokinase, Muscle (PFKM) protein (Myc-DYKDDDDK Tag)PFKM 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- PFKM (phosphofructokinase, Muscle (PFKM))
- 别名
- PFKM (PFKM 产品)
- 别名
- GSD7 Protein, PFK-1 Protein, PFK1 Protein, PFKA Protein, PFKX Protein, AI131669 Protein, PFK-A Protein, PFK-M Protein, Pfk-4 Protein, Pfk4 Protein, Pfka Protein, Pfkx Protein, Pfk-M Protein, M-PFK Protein, PFKM Protein, pfkm Protein, zgc:92344 Protein, pfk Protein, im:7137110 Protein, wu:fc58g11 Protein, phosphofructokinase, muscle Protein, phosphofructokinase, platelet Protein, phosphofructokinase, muscle a Protein, phosphofructokinase, muscle S homeolog Protein, phosphofructokinase, muscle b Protein, PFKM Protein, Pfkm Protein, PFKP Protein, pfkma Protein, pfkm.S Protein, pfkmb Protein
- 背景
- Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
- 分子量
- 86.0 kDa Including tag.
- UniProt
- P08237
- 途径
- Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect
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