PERP Protein (AA 1-193) (rho-1D4 tag)
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- 抗原 See all PERP 蛋白
- PERP (TP53 Apoptosis Effector (PERP))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-193
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宿主
- 人
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资源
- Insect Cells
- 标记
- This PERP protein is labelled with rho-1D4 tag.
- 应用范围
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- 序列
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MIRCGLACER CRWILPLLLL SAIAFDIIAL AGRGWLQSSD HGQTSSLWWK CSQEGGGSGS YEEGCQSLME YAWGRAAAAM LFCGFIILVI CFILSFFALC GPQMLVFLRV IGGLLALAAV FQIISLVIYP VKYTQTFTLH ANPAVTYIYN WAYGFGWAAT IILIGCAFFF CCLPNYEDDL LGNAKPRYFY TSA
Sequence without tag. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human PERP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
- Tag Location: C-terminal Rho1D4-tag
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- The protein is purified from the cleared cell lysate using Rho1D4 capture materials.
- Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- In solution (50 mM Hepes, pH 7.4; 100 mM NaCl; 0,1 % OGNG; 0,01% CHS)
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- PERP (TP53 Apoptosis Effector (PERP))
- 别名
- PERP (PERP 产品)
- 别名
- KCP1 Protein, KRTCAP1 Protein, PIGPC1 Protein, RP3-496H19.1 Protein, THW Protein, dJ496H19.1 Protein, 1110017A08Rik Protein, kcp1 Protein, krtcap1 Protein, pigpc1 Protein, thw Protein, fk24g11 Protein, wu:fk24g11 Protein, PERP Protein, PERP, TP53 apoptosis effector Protein, PERP, TP53 apoptosis effector S homeolog Protein, PERP Protein, Perp Protein, perp.S Protein, perp Protein
- 背景
- Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway (By similarity). {ECO:0000250}.
- 分子量
- 22.6 kDa Including tag.
- UniProt
- Q96FX8
- 途径
- Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity
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