电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

NPTN Protein (AA 29-339) (His tag)

Crystallography grade NPTN 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
产品编号 ABIN3084717
发货至: 中国
  • 抗原 See all NPTN 蛋白
    NPTN (Neuroplastin (NPTN))
    蛋白类型
    Recombinant
    产品特性
    AA 29-339
    宿主
    • 6
    • 2
    • 1
    资源
    • 3
    • 2
    • 2
    • 1
    • 1
    Insect Cells
    标记
    This NPTN protein is labelled with His tag.
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    序列
    MQNAGFVKSP MSETKLTGDA FELYCDVVGS PTPEIQWWYA EVNRAESFRQ LWDGARKRRV TVNTAYGSNG VSVLRITRLT LEDSGTYECR ASNDPKRNDL RQNPSITWIR AQATISVLQK PRIVTSEEVI IRDSPVLPVT LQCNLTSSSH TLTYSYWTKN GVELSATRKN ASNMEYRINK PRAEDSGEYH CVYHFVSAPK ANATIEVKAA PDITGHKRSE NKNEGQDATM YCKSVGYPHP DWIWRKKENG MPMDIVNTSG RFFIINKENY TELNIVNLQI TEDPGEYECN ATNAIGSASV VTVLRVRSHL APHHHHHH
    Sequence including C-terminal His-tag.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human NPTN Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    • Tag Location: C-terminal His Tag

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin-free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product NPTN 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    NPTN (Neuroplastin (NPTN))
    别名
    NPTN (NPTN 产品)
    别名
    SDFR1 Protein, GP55 Protein, GP65 Protein, SDR1 Protein, np55 Protein, np65 Protein, AW554172 Protein, Sdfr1 Protein, nptn Protein, zgc:77326 Protein, neuroplastin Protein, neuroplastin L homeolog Protein, neuroplastin b Protein, NPTN Protein, Nptn Protein, nptn.L Protein, nptnb Protein
    背景
    Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity (By similarity). {ECO:0000250}.
    分子量
    35.0 kD. Including tag.
    UniProt
    Q9Y639
    途径
    Regulation of long-term Neuronal Synaptic Plasticity
You are here:
客服