M6PR Protein (AA 27-185) (His tag)
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- 抗原 See all M6PR 蛋白
- M6PR (Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR))
- 蛋白类型
- Recombinant
- 产品特性
- AA 27-185
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宿主
- 人
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资源
- Insect Cells
- 标记
- This M6PR protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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TEEKTCDLVG EKGKESEKEL ALVKRLKPLF NKSFESTVGQ GSDTYIYIFR VCREAGNHTS GAGLVQINKS NGKETVVGRL NETHIFNGSN WIMLIYKGGD EYDNHCGKEQ RRAVVMISCN RHTLADNFNP VSEERGKVQD CFYLFEMDSS LACSPEISH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human M6PR Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product M6PR 蛋白
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Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR) (AA 211-277) protein (GST tag)
M6PR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR) (AA 22-186) protein (His tag)Crystallography grade M6PR 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR) (AA 27-277) protein (rho-1D4 tag)Crystallography grade M6PR 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR) (AA 22-278) protein (rho-1D4 tag)Crystallography grade M6PR 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR) (AA 2328-2491), (partial) protein (GST tag)M6PR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR) protein (Myc-DYKDDDDK Tag)M6PR 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR) (AA 1-277), (full length) protein (GST tag)M6PR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % ELISA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- M6PR (Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR))
- 别名
- M6PR (M6PR 产品)
- 别名
- CD222 Protein, CIMPR Protein, M6P-R Protein, MPR1 Protein, MPRI Protein, CD-MPR Protein, MPR 46 Protein, MPR-46 Protein, MPR46 Protein, SMPR Protein, Mpr46 Protein, CDMPR Protein, m6pr Protein, MGC68896 Protein, MGC130765 Protein, zgc:77757 Protein, wu:fj81h11 Protein, MGC89423 Protein, M6PR Protein, mprd Protein, C16orf27 Protein, GNPTAG Protein, LP2537 Protein, RJD9 Protein, insulin like growth factor 2 receptor Protein, mannose-6-phosphate receptor, cation dependent Protein, mannose-6-phosphate receptor (cation dependent) L homeolog Protein, mannose-6-phosphate receptor (cation dependent) Protein, mannose-6-phosphate receptor (cation dependent) S homeolog Protein, Cation-dependent mannose-6-phosphate receptor Protein, N-acetylglucosamine-1-phosphate transferase gamma subunit Protein, IGF2R Protein, M6PR Protein, M6pr Protein, m6pr.L Protein, m6pr Protein, m6pr.S Protein, Ethha_1678 Protein, mprd Protein, GNPTG Protein
- 背景
- Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
- 分子量
- 18.8 kDa Including tag.
- UniProt
- P20645
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