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MED4 Protein (AA 2-270) (His tag)

Crystallography grade MED4 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
产品编号 ABIN3083536
发货至: 中国
  • 抗原 See all MED4 蛋白
    MED4 (Mediator Complex Subunit 4 (MED4))
    蛋白类型
    Recombinant
    产品特性
    AA 2-270
    宿主
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    资源
    • 8
    • 2
    • 1
    • 1
    大肠杆菌(E. Coli)
    标记
    This MED4 protein is labelled with His tag.
    应用范围
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    序列
    AASSSGEKEK ERLGGGLGVA GGNSTRERLL SALEDLEVLS RELIEMLAIS RNQKLLQAGE ENQVLELLIH RDGEFQELMK LALNQGKIHH EMQVLEKEVE KRDSDIQQLQ KQLKEAEQIL ATAVYQAKEK LKSIEKARKG AISSEEIIKY AHRISASNAV CAPLTWVPGD PRRPYPTDLE MRSGLLGQMN NPSTNGVNGH LPGDALAAGR LPDVLAPQYP WQSNDMSMNM LPPNHSSDFL LEPPGHNKEN EDDVEIMSTD SSSSSSESD
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human MED4 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product MED4 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    MED4 (Mediator Complex Subunit 4 (MED4))
    别名
    MED4 (MED4 产品)
    别名
    zgc:110646 Protein, ARC36 Protein, DRIP36 Protein, HSPC126 Protein, TRAP36 Protein, VDRIP Protein, Vdrip Protein, 2410046H15Rik Protein, Vdirp Protein, mediator complex subunit 4 Protein, mediator complex subunit 4 S homeolog Protein, med4 Protein, med4.S Protein, MED4 Protein, Med4 Protein
    背景
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
    分子量
    30.6 kDa Including tag.
    UniProt
    Q9NPJ6
    途径
    Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha
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