GPD1 Protein (AA 1-349) (Strep Tag)
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- 抗原 See all GPD1 蛋白
- GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-349
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宿主
- 人
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This GPD1 protein is labelled with Strep Tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
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MASKKVCIVG SGNWGSAIAK IVGGNAAQLA QFDPRVTMWV FEEDIGGKKL TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAAEDADILI FVVPHQFIGK ICDQLKGHLK ANATGISLIK GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVADEK FCETTIGCKD PAQGQLLKEL MQTPNFRITV VQEVDTVEIC GALKNVVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGPVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE LLNGQKLQGP ETARELYSIL QHKGLVDKFP LFMAVYKVCY EGQPVGEFIH CLQNHPEHM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product GPD1 蛋白
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Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) protein (Strep Tag)
Crystallography grade GPD1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) protein (His tag)GPD1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) protein (GST tag)GPD1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349), (full length) protein (His-SUMO Tag)GPD1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) protein (Myc-DYKDDDDK Tag)GPD1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))
- 别名
- GPD1 (GPD1 产品)
- 别名
- GPDH Protein, Gpd3 Protein, GPD-C Protein, GPDH-C Protein, HTGTI Protein, CG9042 Protein, DROGPDHA Protein, DmG3PDH Protein, Dmel\\CG9042 Protein, G3PDH Protein, G3pdh Protein, GAPDH Protein, GPD Protein, GPDA Protein, GPDH-1 Protein, Gdh Protein, Gpd Protein, alpha-GPD Protein, alpha-GPDH Protein, alpha-GPDH-1 Protein, alpha-Gpdh Protein, alphaGPDH Protein, alphaGpd Protein, alphaGpdh Protein, alphaGpdh-1 Protein, gpdh Protein, gpdh-1 Protein, sn-Gpdh Protein, AI747587 Protein, Gdc-1 Protein, Gdc1 Protein, mKIAA4010 Protein, gpd1 Protein, wu:fc30a07 Protein, zgc:63859 Protein, MGC79676 Protein, GPD1 Protein, LG3P Protein, zgc:112197 Protein, Gpd1 Protein, gpd1h Protein, gpd1l Protein, wu:fc58b05 Protein, zgc:66051 Protein, zgc:85742 Protein, glycerol-3-phosphate dehydrogenase 1 Protein, Glycerol-3-phosphate dehydrogenase [NAD(+)] Protein, glycerol-3-phosphate dehydrogenase Protein, Glycerol 3 phosphate dehydrogenase Protein, glycerol-3-phosphate dehydrogenase 1 (soluble) Protein, glycerol-3-phosphate dehydrogenase 1 L homeolog Protein, glycerol-3-phosphate dehydrogenase 1c Protein, glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic Protein, glycerol-3-phosphate dehydrogenase 1a Protein, glycerol-3-phosphate dehydrogenase 1b Protein, Gpd1 Protein, gpdh-1 Protein, GPD1 Protein, Tb11.02.5280 Protein, Gpdh Protein, gpd1.L Protein, gpd1c Protein, gpd1 Protein, LOC101071719 Protein, gpd1a Protein, gpd1b Protein
- 背景
- Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (GPD-C) (GPDH-C) (EC 1.1.1.8),FUNCTION: Has glycerol-3-phosphate dehydrogenase activity. {ECO:0000269|PubMed:7772607}.
- 分子量
- 37.6 kDa
- UniProt
- P21695
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