ECE1 Protein (AA 90-770) (His tag)
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- 抗原 See all ECE1 蛋白
- ECE1 (Endothelin-Converting Enzyme 1 (ECE1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 90-770
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宿主
- 人
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资源
- Insect Cells
- 标记
- This ECE1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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QYQTRSPSVC LSEACVSVTS SILSSMDPTV DPCHDFFSYA CGGWIKANPV PDGHSRWGTF SNLWEHNQAI IKHLLENSTA SVSEAERKAQ VYYRACMNET RIEELRAKPL MELIERLGGW NITGPWAKDN FQDTLQVVTA HYRTSPFFSV YVSADSKNSN SNVIQVDQSG LGLPSRDYYL NKTENEKVLT GYLNYMVQLG KLLGGGDEEA IRPQMQQILD FETALANITI PQEKRRDEEL IYHKVTAAEL QTLAPAINWL PFLNTIFYPV EINESEPIVV YDKEYLEQIS TLINTTDRCL LNNYMIWNLV RKTSSFLDQR FQDADEKFME VMYGTKKTCL PRWKFCVSDT ENNLGFALGP MFVKATFAED SKSIATEIIL EIKKAFEESL STLKWMDEET RKSAKEKADA IYNMIGYPNF IMDPKELDKV FNDYTAVPDL YFENAMRFFN FSWRVTADQL RKAPNRDQWS MTPPMVNAYY SPTKNEIVFP AGILQAPFYT RSSPKALNFG GIGVVVGHEL THAFDDQGRE YDKDGNLRPW WKNSSVEAFK RQTECMVEQY SNYSVNGEPV NGRHTLGENI ADNGGLKAAY RAYQNWVKKN GAEHSLPTLG LTNNQLFFLG FAQVWCSVRT PESSHEGLIT DPHSPSRFRV IGSLSNSKEF SEHFRCPPGS PMNPPHKCEV W
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ECE1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ECE1 蛋白
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Endothelin-Converting Enzyme 1 (ECE1) (AA 1-770) protein (GST tag)
ECE1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Endothelin-Converting Enzyme 1 (ECE1) (AA 1-770) protein (Strep Tag)Crystallography grade ECE1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Endothelin-Converting Enzyme 1 (ECE1) (AA 89-769) protein (His tag)Crystallography grade ECE1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Endothelin-Converting Enzyme 1 (ECE1) (AA 1-769) protein (Strep Tag)Crystallography grade ECE1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Endothelin-Converting Enzyme 1 (ECE1) proteinECE1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- ECE1 (Endothelin-Converting Enzyme 1 (ECE1))
- 别名
- ECE1 (ECE1 产品)
- 别名
- ECE Protein, Ece Protein, AW322500 Protein, ECE-1 Protein, ECE-1a Protein, ECE-1b Protein, ECE-1d Protein, ece1 Protein, MGC52874 Protein, MGC130791 Protein, fd11b07 Protein, wu:fd11b07 Protein, zgc:154079 Protein, endothelin converting enzyme 1 Protein, endothelin converting enzyme like 1 Protein, endothelin converting enzyme 1 L homeolog Protein, endothelin-converting enzyme 1 Protein, Endothelin-converting enzyme 1 Protein, ECE1 Protein, ECEL1 Protein, Ece1 Protein, ece1.L Protein, Za10_0551 Protein, Arch_0474 Protein, Closa_0961 Protein, Odosp_0578 Protein, Bacsa_1787 Protein, Weevi_2017 Protein, Fluta_3444 Protein, Sphch_2390 Protein, ece1 Protein
- 背景
- Converts big endothelin-1 to endothelin-1. {ECO:0000269|PubMed:9396733}.
- 分子量
- 78.6 kDa Including tag.
- UniProt
- P42892
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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