eEF1A1 Protein (AA 1-462) (Strep Tag)
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- 抗原 See all eEF1A1 (EEF1A1) 蛋白
- eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-462
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宿主
- 人
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This eEF1A1 protein is labelled with Strep Tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
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MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK VTKSAQKAQK AK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product EEF1A1 蛋白
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Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (AA 1-462) protein (GST tag)
EEF1A1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (AA 156-255) protein (GST tag)EEF1A1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (AA 1-462) protein (Strep Tag)Crystallography grade EEF1A1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) protein (Myc-DYKDDDDK Tag)EEF1A1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (AA 1-462) proteinEEF1A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))
- 别名
- EEF1A1 (EEF1A1 产品)
- 别名
- CCS-3 Protein, CCS3 Protein, EE1A1 Protein, EEF-1 Protein, EEF1A Protein, EF-Tu Protein, EF1A Protein, GRAF-1EF Protein, HNGC:16303 Protein, LENG7 Protein, PTI1 Protein, eEF1A-1 Protein, Eef1a2 Protein, Eef1a2l1 Protein, SI Protein, EEF1A2 Protein, EF1A1 Protein, eef1a1 Protein, wu:fj34g08 Protein, zgc:110335 Protein, EEF1A1 Protein, RABEFLA2 Protein, EFL1-alpha Protein, chunp6927 Protein, eef1a Protein, ef1a Protein, ik:tdsubc_2a3 Protein, ik:tdsubc_2b3 Protein, tdsubc_2a3 Protein, wu:fa91c07 Protein, wu:fa94b03 Protein, wu:fi13b09 Protein, xx:tdsubc_2a3 Protein, xx:tdsubc_2b3 Protein, EF-1A Protein, EF-1-ALPHA-S Protein, eef1a-s Protein, eef1as Protein, fj64c02 Protein, wu:fj64c02 Protein, zgc:73138 Protein, eukaryotic translation elongation factor 1 alpha 1 Protein, eukaryotic translation elongation factor 1 alpha 1b Protein, eukaryotic translation elongation factor 1 alpha 1, like 1 Protein, eukaryotic translation elongation factor 1 alpha 1 L homeolog Protein, elongation factor 1-alpha Protein, eukaryotic translation elongation factor 1 alpha 1a Protein, EEF1A1 Protein, Eef1a1 Protein, eef1a1b Protein, eef1a1l1 Protein, eef1a1.L Protein, tufA Protein, eef1a1a Protein
- 背景
- Elongation factor 1-alpha 1 (EF-1-alpha-1) (EC 3.6.5.-) (Elongation factor Tu) (EF-Tu) (Eukaryotic elongation factor 1 A-1) (eEF1A-1) (Leukocyte receptor cluster member 7),FUNCTION: Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis (PubMed:26651998, PubMed:26593721, PubMed:36264623, PubMed:36123449, PubMed:36638793). Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome (PubMed:26651998, PubMed:26593721, PubMed:36264623, PubMed:36123449, PubMed:36638793). The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation (PubMed:26651998, PubMed:26593721, PubMed:36264623, PubMed:36123449). Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed:17177976). {ECO:0000269|PubMed:17177976, ECO:0000269|PubMed:26593721, ECO:0000269|PubMed:26651998, ECO:0000269|PubMed:36123449, ECO:0000269|PubMed:36264623, ECO:0000269|PubMed:36638793}., FUNCTION: (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection. {ECO:0000269|PubMed:33495306}.
- 分子量
- 50.1 kDa
- UniProt
- P68104
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