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COPZ1 Protein (AA 1-177) (His tag)

Crystallography grade COPZ1 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
产品编号 ABIN3078073
发货至: 中国
  • 抗原 See all COPZ1 蛋白
    COPZ1 (Coatomer Protein Complex, Subunit zeta 1 (COPZ1))
    蛋白类型
    Recombinant
    产品特性
    AA 1-177
    宿主
    • 3
    • 1
    • 1
    • 1
    资源
    • 2
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    大肠杆菌(E. Coli)
    标记
    This COPZ1 protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    序列
    MEALILEPSL YTVKAILILD NDGDRLFAKY YDDTYPSVKE QKAFEKNIFN KTHRTDSEIA LLEGLTVVYK SSIDLYFYVI GSSYENELML MAVLNCLFDS LSQMLRKNVE KRALLENMEG LFLAVDEIVD GGVILESDPQ QVVHRVALRG EDVPLTEQTV SQVLQSAKEQ IKWSLLR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human COPZ1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product COPZ1 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    COPZ1 (Coatomer Protein Complex, Subunit zeta 1 (COPZ1))
    别名
    COPZ1 (COPZ1 产品)
    别名
    COPZ Protein, T7P1.11 Protein, T7P1_11 Protein, HSPC181 Protein, zeta-COP Protein, zeta1-COP Protein, 5930435A22Rik Protein, AA407760 Protein, D4Ertd360e Protein, coatomer protein complex, subunit zeta 1 Protein, coatomer protein complex subunit zeta 1 Protein, Coatomer protein complex, subunit zeta 1 Protein, SNARE-like superfamily protein Protein, coatomer subunit zeta-1 Protein, Copz1 Protein, COPZ1 Protein, CopZ Protein, copz1 Protein, AT1G60970 Protein, CpipJ_CPIJ003005 Protein, Tsp_01470 Protein
    背景
    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins, the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). {ECO:0000250}., The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.
    分子量
    21.2 kDa Including tag.
    UniProt
    P61923
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