GM2A Protein (AA 32-193) (His tag)
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- 抗原 See all GM2A 蛋白
- GM2A (GM2 Ganglioside Activator (GM2A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 32-193
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宿主
- 人
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资源
- Insect Cells
- 标记
- This GM2A protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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SSFSWDNCDE GKDPAVIRSL TLEPDPIIVP GNVTLSVMGS TSVPLSSPLK VDLVLEKEVA GLWIKIPCTD YIGSCTFEHF CDVLDMLIPT GEPCPEPLRT YGLPCHCPFK EGTYSLPKSE FVVPDLELPS WLTTGNYRIE SVLSSSGKRL GCIKIAASLK GI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human GM2A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product GM2A 蛋白
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GM2 Ganglioside Activator (GM2A) (AA 26-193) protein (His tag)
GM2A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
GM2 Ganglioside Activator (GM2A) (AA 1-193) protein (GST tag)GM2A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
GM2 Ganglioside Activator (GM2A) (AA 94-193) protein (GST tag)GM2A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
GM2 Ganglioside Activator (GM2A) (AA 34-193) protein (His tag)Crystallography grade GM2A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
GM2 Ganglioside Activator (GM2A) (AA 21-193) protein (His tag)Crystallography grade GM2A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- GM2A (GM2 Ganglioside Activator (GM2A))
- 别名
- GM2A (GM2A 产品)
- 别名
- GM2A Protein, fb96e04 Protein, fb96e10 Protein, wu:fb96e04 Protein, wu:fb96e10 Protein, zgc:110188 Protein, MGC84154 Protein, GM2-AP Protein, SAP-3 Protein, AA408702 Protein, AW215435 Protein, GM2 ganglioside activator Protein, GM2 ganglioside activator L homeolog Protein, GM2 ganglioside activator protein Protein, Gm2a Protein, GM2A Protein, gm2a Protein, gm2a.L Protein
- 背景
- The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity (By similarity). Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 Molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. {ECO:0000250}.
- 分子量
- 18.5 kDa Including tag.
- UniProt
- P17900
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