QTRT1 Protein (AA 2-403) (His tag)
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- 抗原 See all QTRT1 蛋白
- QTRT1 (Queuine tRNA-Ribosyltransferase 1 (QTRT1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-403
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宿主
- 人
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资源
- Insect Cells
- 标记
- This QTRT1 protein is labelled with His tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- 序列
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AGAATQASLE SAPRIMRLVA ECSRSRARAG ELWLPHGTVA TPVFMPVGTQ ATMKGITTEQ LDALGCRICL GNTYHLGLRP GPELIQKANG LHGFMNWPHN LLTDSGGFQM VSLVSLSEVT EEGVRFRSPY DGNETLLSPE KSVQIQNALG SDIIMQLDDV VSSTVTGPRV EEAMYRSIRW LDRCIAAHQR PDKQNLFAII QGGLDADLRA TCLEEMTKRD VPGFAIGGLS GGESKSQFWR MVALSTSRLP KDKPRYLMGV GYATDLVVCV ALGCDMFDCV FPTRTARFGS ALVPTGNLQL RKKVFEKDFG PIDPECTCPT CQKHSRAFLH ALLHSDNTAA LHHLTVHNIA YQLQLMSAVR TSIVEKRFPD FVRDFMGAMY GDPTLCPTWA TDALASVGIT LG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human QTRT1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product QTRT1 蛋白
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Queuine tRNA-Ribosyltransferase 1 (QTRT1) (AA 1-358) protein (GST tag)
QTRT1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Queuine tRNA-Ribosyltransferase 1 (QTRT1) (AA 2-403) protein (His tag)Crystallography grade QTRT1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- QTRT1 (Queuine tRNA-Ribosyltransferase 1 (QTRT1))
- 别名
- QTRT1 (QTRT1 产品)
- 别名
- CG4947 Protein, Dmel\\CG4947 Protein, TGT Protein, tgt Protein, zgc:66378 Protein, FP3235 Protein, TGUT Protein, 2610028E17Rik Protein, Tgt Protein, Tgut Protein, tRNA-guanine transglycosylase Protein, queuine tRNA-ribosyltransferase 1 S homeolog Protein, queuine tRNA-ribosyltransferase 1 Protein, queuine tRNA-ribosyltransferase catalytic subunit 1 Protein, Tgt Protein, Olsu_0732 Protein, Palpr_1298 Protein, Riean_1227 Protein, Calni_1240 Protein, Ftrac_0312 Protein, Ocepr_1541 Protein, Sulku_0977 Protein, Tmar_0854 Protein, Bache_2323 Protein, Nitsa_1291 Protein, Isop_1657 Protein, Despr_0021 Protein, Pedsa_1842 Protein, Corgl_1148 Protein, Mahau_0921 Protein, Theth_0831 Protein, qtrt1.S Protein, qtrt1 Protein, QTRT1 Protein, Qtrt1 Protein
- 背景
- Interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity). {ECO:0000250}.
- 分子量
- 44.9 kDa Including tag.
- UniProt
- Q9BXR0
- 途径
- Ribonucleoside Biosynthetic Process
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