SUMO2 Protein (AA 1-93) (His tag)
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- 抗原 See all SUMO2 蛋白
- SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-93
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This SUMO2 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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MADEKPKEGV KTENNDHINL KVAGQDGSVV QFKIKRHTPL SKLMKAYCER QGLSMRQIRF RFDGQPINET DTPAQLEMED EDTIDVFQQQ TGG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SUMO2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product SUMO2 蛋白
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Small Ubiquitin Related Modifier 2 (SUMO2) (AA 1-93) protein (His tag)
SUMO2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Small Ubiquitin Related Modifier 2 (SUMO2) (AA 1-93) protein (His-IF2DI Tag)SUMO2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Small Ubiquitin Related Modifier 2 (SUMO2) (AA 1-95) protein (GST tag)SUMO2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Small Ubiquitin Related Modifier 2 (SUMO2) (AA 1-93) protein (His tag)Crystallography grade SUMO2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Small Ubiquitin Related Modifier 2 (SUMO2) (Transcript Variant 2) protein (His tag)SUMO2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Small Ubiquitin Related Modifier 2 (SUMO2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)SUMO2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Small Ubiquitin Related Modifier 2 (SUMO2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SUMO2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))
- 别名
- SUMO2 (SUMO2 产品)
- 别名
- HSMT3 Protein, SMT3B Protein, SMT3H2 Protein, SUMO3 Protein, Smt3A Protein, SMT3A Protein, SMT3H1 Protein, SUMO-3 Protein, Smt3B Protein, Smt3h2 Protein, sumo2 Protein, zgc:92241 Protein, SUMO2 Protein, SUMO-2 Protein, Smt3b Protein, hsmt3 Protein, smt3b Protein, smt3h2 Protein, sumo-2 Protein, PSMT3 Protein, ATSUMO2 Protein, MCO15.11 Protein, MCO15_11 Protein, SMALL UBIQUITIN-LIKE MODIFIER 2 Protein, SUM2 Protein, SUMO 2 Protein, small ubiquitin-like modifier 2 Protein, small ubiquitin-like modifier 2 Protein, small ubiquitin-like modifier 3 Protein, small ubiquitin-like modifier 2b Protein, small ubiquitin-related modifier 2 Protein, small ubiquitin-like modifier 2 S homeolog Protein, small ubiquitin-like modifier 2 L homeolog Protein, SUMO2 Protein, SUMO3 Protein, Sumo2 Protein, sumo2b Protein, LOC714892 Protein, sumo2.S Protein, sumo2.L Protein
- 背景
- Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744). {ECO:0000269|PubMed:18408734, ECO:0000269|PubMed:18538659, ECO:0000269|PubMed:21965678, ECO:0000269|PubMed:24105744, ECO:0000269|PubMed:26524494, ECO:0000269|PubMed:9556629}.
- 分子量
- 11.6 kDa Including tag.
- UniProt
- P61956
- 途径
- Methionine Biosynthetic Process
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