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PD-L1 Protein (AA 19-238) (His tag,AVI tag,Biotin)

PD-L1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
产品编号 ABIN2870558
发货至: 中国
  • 抗原 See all PD-L1 蛋白
    PD-L1 (CD274 (PD-L1))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 19-238
    宿主
    • 41
    • 18
    • 6
    • 5
    • 4
    • 3
    • 1
    资源
    • 46
    • 9
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    标记
    This PD-L1 protein is labelled with His tag,AVI tag,Biotin.
    品牌
    MABSol®,PrecisionAvi
    序列
    AA 19-238
    特异性
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    产品特性
    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 27.8 kDa. The protein migrates as 38-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    纯度
    >95 % as determined by SDS-PAGE.
    内毒素水平
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product PD-L1 蛋白
  • 说明

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    PBS, pH 7.4
    注意事项
    Please avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
  • Son, Park, Seo, Lee, Heo, Kim: "A small-sized protein binder specific for human PD-1 effectively suppresses the tumour growth in tumour mouse model." in: Journal of drug targeting, pp. 1-9, (2019) (PubMed).

    Ganesan, Ahmed, Okoye, Arutyunova, Babu, Turnbull, Kundu, Shields, Agopsowicz, Xu, Tabana, Srivastava, Zhang, Moon, Belovodskiy, Hena, Kandadai, Hosseini, Hitt, Walker, Smylie, West, Siraki, Lemieux et al.: "Comprehensive in vitro characterization of PD-L1 small molecule inhibitors. ..." in: Scientific reports, Vol. 9, Issue 1, pp. 12392, (2019) (PubMed).

    Arlauckas, Garris, Kohler, Kitaoka, Cuccarese, Yang, Miller, Carlson, Freeman, Anthony, Weissleder, Pittet: "In vivo imaging reveals a tumor-associated macrophage-mediated resistance pathway in anti-PD-1 therapy." in: Science translational medicine, Vol. 9, Issue 389, (2018) (PubMed).

  • 抗原
    PD-L1 (CD274 (PD-L1))
    别名
    PD-L1 (PD-L1 产品)
    别名
    B7-H Protein, B7H1 Protein, PD-L1 Protein, PDCD1L1 Protein, PDCD1LG1 Protein, PDL1 Protein, A530045L16Rik Protein, B7h1 Protein, Pdcd1l1 Protein, Pdcd1lg1 Protein, Pdl1 Protein, RGD1566211 Protein, CD274 molecule Protein, CD274 antigen Protein, programmed cell death 1 ligand 2 Protein, CD274 Protein, Cd274 Protein, PDCD1LG2 Protein
    背景
    Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
    分子量
    27.8 kDa
    NCBI登录号
    NP_054862
    途径
    Cancer Immune Checkpoints
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