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TNF alpha 蛋白

TNF alpha 宿主: 大鼠 宿主: 大肠杆菌(E. Coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining Func, STD, AbP, PI Active
产品编号 ABIN2734614
发货至: 中国
  • 抗原 See all TNF alpha 蛋白
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    蛋白类型
    Recombinant
    生物活性
    Active
    宿主
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    大鼠
    资源
    • 58
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    大肠杆菌(E. Coli)
    应用范围
    Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
    特异性
    Optimal preservation of protein structure, post-translational modifications and functions.
    产品特性
    • Recombinant human Tumor necrosis factor (TNF-alpha) protein expressed in E. coli.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.
    纯度
    > 95 % as determined by SDS-PAGE and Coomassie blue staining
    内毒素水平
    Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
    Biological Activity Comment
    ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is less than or equal to 0.05 ng/ml, corresponding to a specific activity of > 2 x 10^7 units/mg.
    Top Product
    Discover our top product TNF alpha 蛋白
  • 应用备注
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays
    限制
    仅限研究用
  • 缓冲液
    Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
    注意事项
    Resuspend the protein in the desired concentration in proper buffer
    储存条件
    -80 °C
    储存方法
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • 抗原
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    别名
    Tumor Necrosis Factor (Tnf-Alpha) (TNF alpha 产品)
    别名
    DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein
    分子量
    17.3 kDa
    NCBI登录号
    NP_036807
    途径
    NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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