PTPN1 Protein (His tag)
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- 抗原 See all PTPN1 蛋白
- PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PTPN1 protein is labelled with His tag.
- 应用范围
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human PTPN1 protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 90 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- Specific activity: ~14000 nmol/min/mg. Enzymatic activity was determined by measuring the amount of 4-Nitrophenol hydrolyzed from p-nitrophenyl phosphate (pNPP) at 30C.
- Top Product
- Discover our top product PTPN1 蛋白
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Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 268-406) protein (His tag)
PTPN1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 1-321) (Active) proteinPTPN1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 1-435) protein (GST tag)PTPN1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 322-406) protein (GST tag)PTPN1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (Active) protein (His tag)PTPN1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 1-435) protein (Strep Tag)Crystallography grade PTPN1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 1-432) protein (Strep Tag)Crystallography grade PTPN1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) protein (DYKDDDDK Tag)PTPN1 宿主: 人 宿主: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) protein (Myc-DYKDDDDK Tag)PTPN1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the N-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM HEPES, 50 mM NaCl, 2.5 mM EDTA, PH7.5,5 mM DTT. < td valign=top>
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))
- 别名
- Ptpn1 (PTPN1 产品)
- 别名
- PTPN1 Protein, PTP1B Protein, PTP-1B Protein, PTP-HA2 Protein, Ptp Protein, ptp1b Protein, wu:fk54h03 Protein, CPTP1 Protein, protein tyrosine phosphatase, non-receptor type 1 Protein, protein tyrosine phosphatase, non-receptor type 1 L homeolog Protein, PTPN1 Protein, Ptpn1 Protein, ptpn1 Protein, ptpn1.L Protein
- 背景
- The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.
- 分子量
- 39.2 KDa
- NCBI登录号
- NP_002818
- 途径
- TLR signaling, Response to Growth Hormone Stimulus, ER-Nucleus Signaling, Platelet-derived growth Factor Receptor Signaling
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