PPM1D Protein (Myc-DYKDDDDK Tag)
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- PPM1D (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D (PPM1D))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This PPM1D protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human Protein phosphatase 1D / PPM1D protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Discover our top product PPM1D 蛋白
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- PPM1D (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D (PPM1D))
- 别名
- Protein Phosphatase 1d,ppm1d (PPM1D 产品)
- 背景
- The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.
- 分子量
- 66.5 kDa
- NCBI登录号
- NP_003611
- 途径
- p53 Pathway, Cell Division Cycle
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