IFNB1 蛋白
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- 抗原 See all IFNB1 蛋白
- IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- CHO Cells
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human IFNB / Interferon beta protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Assay #1:Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/ml. Assay #2:Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is less than or equal to 0.1 ng/ml, corresponding to a specific activity of > 1 x 10^7 units/mg
- Top Product
- Discover our top product IFNB1 蛋白
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Interferon, beta 1, Fibroblast (IFNB1) (AA 102-187) protein (GST tag)
IFNB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interferon, beta 1, Fibroblast (IFNB1) (AA 22-182) protein (His tag)IFNB1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Interferon, beta 1, Fibroblast (IFNB1) (AA 1-187) protein (GST tag)IFNB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interferon, beta 1, Fibroblast (IFNB1) (AA 22-187), (full length) protein (GST tag)IFNB1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Centrifuge the vial prior to opening. Reconstitute the protein in water to a concnetration of 0.1 ~ 1.0 mg/mL. Do not vertex. For extended storage, it is recommended to further dilute in a proper buffer (i.e. water or PBS) containing a carrier protein such as 0.1 % BSA, and store in working aliquots at -20 °C or -80 °C
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))
- 别名
- Ifnb,interferon beta (IFNB1 产品)
- 别名
- IFB Protein, IFF Protein, IFNB Protein, IFN-beta Protein, Ifb Protein, Ifnb Protein, IFNb Protein, interferon beta 1 Protein, interferon beta 1, fibroblast Protein, interferon, beta 1, fibroblast Protein, IFNB1 Protein, Ifnb1 Protein
- 背景
- Has antiviral, antibacterial and anticancer activities. [UniProtKB/Swiss-Prot Function]
- 分子量
- 20 kDa
- NCBI登录号
- NP_002167
- 途径
- JAK/STAT Signaling, TCR Signaling, TLR signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome
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