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IDO1 Protein (Myc-DYKDDDDK Tag)

IDO1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
产品编号 ABIN2723258
发货至: 中国
  • 抗原 See all IDO1 蛋白
    IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))
    蛋白类型
    Recombinant
    生物活性
    Active
    宿主
    • 9
    • 4
    • 1
    资源
    • 8
    • 2
    • 2
    • 2
    HEK-293 Cells
    标记
    This IDO1 protein is labelled with Myc-DYKDDDDK Tag.
    应用范围
    Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
    特异性
    Optimal preservation of protein structure, post-translational modifications and functions.
    产品特性
    • Recombinant human IDO1 / INDO protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.
    纯度
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
    Biological Activity Comment
    The specific activity of IDOI was determined by monitoring kynurenine formation from N-formylkynurenine based on the absorbance at 492nm. The N-formylkynurenine was produced from a conversion of tryptophan with IDO1. The reaction was carried out at 25°C for 15min in the buffer containing 100mM PBS, pH6.5, 40mM ascorbic acid, 450 units catalase, 20μM methylene blue, and 800μM L-tryptophan as the substrate. The reaction was terminated by adding 50ul of 30% (w/v) trichloroacetic acid. The sample was further incubated for 30min at 60°C and centrifuged at 12000 rpm for 15 min. The supernatant was used to mix with an equal volume of Ehrlich's reagent (2% p-dimethylaminobenzaldehyde in glacial acetic acid) to measure the absorbance at 492 nm after 10min incubation.
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    Discover our top product IDO1 蛋白
  • 应用备注
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays
    说明

    The tag is located at the C-terminal.

    限制
    仅限研究用
  • 浓度
    > 50 μg/mL
    缓冲液
    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
    储存条件
    -80 °C
    储存方法
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • 抗原
    IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))
    别名
    Ido1,indo (IDO1 产品)
    别名
    INDO Protein, ido Protein, indo Protein, IDO Protein, cd107b Protein, IDO1 Protein, IDO-1 Protein, Ido Protein, Indo Protein, indoleamine 2,3-dioxygenase 1 Protein, indoleamine 2,3-dioxygenase 1 L homeolog Protein, indoleamine 2,3-dioxygenase 1-like Protein, IDO1 Protein, ido1.L Protein, ido1 Protein, Ido1 Protein, LOC100050681 Protein
    背景
    This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011].
    分子量
    45.1 kDa
    NCBI登录号
    NP_002155
    途径
    Activated T Cell Proliferation
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