FBLN3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- 抗原 See all FBLN3 蛋白
- FBLN3 (Fibulin 3 (FBLN3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This FBLN3 protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- : 200 ng of recombinant purified EFEMP1-myc were added to cultured glioma cells (CNS-1 rat glioma cell line) for 6h, and cells were further processed for quantitative RT-PCR. Results showed increased expression of metalloproteases MMP2 and MMP9, in agreement with published results from Hu. et. al. (Mol. Cancer Res. (2009) 7:1756-1770) Data kindly provided by Dr. Mariano S. Viapiano, Ohio State University.
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- Discover our top product FBLN3 蛋白
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Fibulin 3 (FBLN3) (AA 18-493) protein (His tag)
FBLN3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibulin 3 (FBLN3) (AA 1-493) protein (GST tag)FBLN3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fibulin 3 (FBLN3) (AA 18-493) protein (His tag)FBLN3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Fibulin 3 (FBLN3) (AA 18-493) protein (His tag)Crystallography grade FBLN3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibulin 3 (FBLN3) (AA 18-493) protein (His tag)Crystallography grade FBLN3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibulin 3 (FBLN3) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)FBLN3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Novel paracrine modulation of Notch-DLL4 signaling by fibulin-3 promotes angiogenesis in high-grade gliomas." in: Cancer research, Vol. 74, Issue 19, pp. 5435-48, (2014) (PubMed).
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: "Novel paracrine modulation of Notch-DLL4 signaling by fibulin-3 promotes angiogenesis in high-grade gliomas." in: Cancer research, Vol. 74, Issue 19, pp. 5435-48, (2014) (PubMed).
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- 抗原
- FBLN3 (Fibulin 3 (FBLN3))
- 别名
- Egf-Containing Fibulin-Like Extracellular Matrix Protein 1 (Efemp1) (FBLN3 产品)
- 别名
- DHRD Protein, DRAD Protein, FBLN3 Protein, FBNL Protein, FIBL-3 Protein, MLVT Protein, MTLV Protein, S1-5 Protein, T16 Protein, EGF containing fibulin like extracellular matrix protein 1 Protein, EGF-containing fibulin-like extracellular matrix protein 1 Protein, epidermal growth factor-containing fibulin-like extracellular matrix protein 1 Protein, EFEMP1 Protein, Efemp1 Protein
- 背景
- This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]
- 分子量
- 52.7 kDa
- NCBI登录号
- NP_004096
- 途径
- EGFR Signaling Pathway
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