ADD2 Protein (Myc-DYKDDDDK Tag)
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- 抗原 See all ADD2 蛋白
- ADD2 (Adducin 2 (Beta) (ADD2))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This ADD2 protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human Beta-adducin (ADD2) (transcript variant beta-4) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ADD2 蛋白
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Adducin 2 (Beta) (ADD2) (AA 1-726) protein (GST tag)
ADD2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Adducin 2 (Beta) (ADD2) (AA 1-726) protein (Strep Tag)Crystallography grade ADD2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Adducin 2 (Beta) (ADD2) (AA 2-725) protein (His tag)Crystallography grade ADD2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- ADD2 (Adducin 2 (Beta) (ADD2))
- 别名
- beta-Adducin (Add2) (ADD2 产品)
- 别名
- si:dkey-202n14.5 Protein, ADDB Protein, 2900072M03Rik Protein, add97 Protein, beta-ADD Protein, adducin 2 Protein, adducin 2 (beta) Protein, ADD2 Protein, add2 Protein, Add2 Protein
- 背景
- Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described.
- 分子量
- 72.5 kDa
- NCBI登录号
- NP_059522
- 途径
- Regulation of Actin Filament Polymerization
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