BRAF Protein (Myc-DYKDDDDK Tag)
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- 抗原 See all BRAF 蛋白
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This BRAF protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human B-Raf proto-oncogene protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product BRAF 蛋白
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B-Raf proto-oncogene, serine/threonine kinase (BRAF) (Val600Glu-Mutant) (Active) protein (GST tag)
BRAF 宿主: 人 宿主: Insect Cells Recombinant SDS, Func Active
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 433-726) (Active) protein (GST tag)BRAF 宿主: 人 宿主: Insect Cells Recombinant SDS, Func Active
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 417-766) (Active) protein (His-GST)BRAF 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (Val600Glu-Mutant) (Active) protein (His-GST)BRAF 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (Active) protein (Myc-DYKDDDDK Tag)BRAF 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, STD, PI Active
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 8-170) protein (His-IF2DI Tag)BRAF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 346-445) protein (GST tag)BRAF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 2-766) protein (His tag)Crystallography grade BRAF 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 2-804) protein (His tag)Crystallography grade BRAF 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
B-Raf proto-oncogene, serine/threonine kinase (BRAF) (Active) protein (DYKDDDDK Tag)BRAF 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, STD, PI Active
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
- 别名
- B-Raf Proto-Oncogene (BRAF 产品)
- 别名
- B-RAF1 Protein, BRAF1 Protein, NS7 Protein, RAFB1 Protein, C-RMIL Protein, RMIL Protein, B-Raf Protein, braf1 Protein, rafb1 Protein, b-raf1 Protein, sierrabraf Protein, BRAF Protein, 9930012E13Rik Protein, AA120551 Protein, AA387315 Protein, AA473386 Protein, B-raf Protein, Braf-2 Protein, Braf2 Protein, C230098H17 Protein, C87398 Protein, D6Ertd631e Protein, braf Protein, fc48g01 Protein, wu:fc48g01 Protein, B-Raf proto-oncogene, serine/threonine kinase Protein, B-Raf proto-oncogene, serine/threonine kinase S homeolog Protein, Braf transforming gene Protein, si:dkey-222f8.3 Protein, BRAF Protein, Braf Protein, braf.S Protein, braf Protein, si:dkey-222f8.3 Protein
- 背景
- This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
- 分子量
- 84 kDa
- NCBI登录号
- NP_004324
- 途径
- MAPK Pathway, RTK signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
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