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- 抗原 See all LSD1 (KDM1A) 蛋白
- LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus
- 标记
- This LSD1 protein is labelled with DYKDDDDK Tag.
- 应用范围
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- 产品特性
- Recombinant LSD1 / KDM1A (accession number NP_055828) was expressed in Sf9 cells and contains an N-terminal FLAG tag with a molecular weight of 97.1 kDa.
- Top Product
- Discover our top product KDM1A 蛋白
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Lysine (K)-Specific Demethylase 1A (KDM1A) (AA 1-876) protein (GST tag)
KDM1A 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Lysine (K)-Specific Demethylase 1A (KDM1A) (AA 753-851) protein (GST tag)KDM1A 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Lysine (K)-Specific Demethylase 1A (KDM1A) (AA 1-852) protein (Strep Tag)Crystallography grade KDM1A 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Lysine (K)-Specific Demethylase 1A (KDM1A) (AA 1-853) protein (Strep Tag)Crystallography grade KDM1A 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Lysine (K)-Specific Demethylase 1A (KDM1A) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)KDM1A 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Recombinant LSD1 / KDM1A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me2 and H3K4me1 demethylase. Histone Demethylase Assay Conditions: 50 mM TrisCl pH 8.25, 0.02 % Triton X100, 10 nM FAD, 5 nM Recombinant LSD1 / KDM1A protein, and 3.3 μM H3K4me2 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- 限制
- 仅限研究用
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- 注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 储存条件
- -80 °C
- 储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- 抗原
- LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))
- 别名
- LSD1 / KDM1A (KDM1A 产品)
- 别名
- AOF2 Protein, BHC110 Protein, KDM1 Protein, LSD1 Protein, 1810043O07Rik Protein, AA408884 Protein, Aof2 Protein, D4Ertd478e Protein, Kdm1 Protein, Lsd1 Protein, mKIAA0601 Protein, DKFZp459N2450 Protein, RGD1562975 Protein, aof2 Protein, kdm1 Protein, wu:fb82e06 Protein, wu:fc51a02 Protein, wu:fj36a05 Protein, wu:fj66b06 Protein, KDM1A Protein, lsd1 Protein, xLSD1 Protein, AtLSD1 Protein, F9F13.30 Protein, F9F13_30 Protein, LESION SIMULATING DISEASE Protein, LESION SIMULATING DISEASE 1 Protein, lysine demethylase 1A Protein, lysine (K)-specific demethylase 1A Protein, lysine (K)-specific demethylase 1a Protein, LSD1 zinc finger family protein Protein, KDM1A Protein, Kdm1a Protein, kdm1a Protein, LSD1 Protein
- 背景
- LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.
- 分子量
- 97.1 kDa
- 途径
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effect
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