CXCL7 Protein (AA 48-109)
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- 抗原 See all CXCL7 (PPBP) 蛋白
- CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 48-109
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 98 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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- Discover our top product PPBP 蛋白
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Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) (AA 35-128) protein
PPBP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) (AA 35-128) protein (His tag)PPBP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) (AA 1-128) protein (GST tag)PPBP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) (AA 37-128) protein (GST tag)PPBP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) (AA 44-124) protein (His tag)Crystallography grade PPBP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) (AA 44-126) protein (His tag)Crystallography grade PPBP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) (AA 44-128) protein (His tag)Crystallography grade PPBP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) (AA 48-128) protein (His tag)Crystallography grade PPBP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) (AA 55-128) protein (His tag)Crystallography grade PPBP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) (AA 56-128) protein (His tag)Crystallography grade PPBP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Bioactivity was measured by its property to chemoattract human neutrophils in a dose dependent manner.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
- 别名
- CXCL7 (PPBP 产品)
- 别名
- 2400003M24Rik Protein, AI854500 Protein, CTAP3 Protein, CTAPIII Protein, Cxcl7 Protein, LA-PF4 Protein, LDGF Protein, MDGF Protein, NAP-2 Protein, NAP-2-L1 Protein, Scyb7 Protein, TGB Protein, TGB1 Protein, THBGB1 Protein, b-TG1 Protein, beta-TG Protein, B-TG1 Protein, Beta-TG Protein, CTAP-III Protein, CXCL7 Protein, PBP Protein, SCYB7 Protein, TC1 Protein, TC2 Protein, THBGB Protein, Nap-2 Protein, CTAPIII/NAP-2 Protein, Platelet basic protein Protein, pro-platelet basic protein Protein, scyb7 Protein, Ppbp Protein, PPBP Protein
- 背景
- CXCL7 is an ERL+ chemokine that is secreted by activated platelets, neutrophils, lymphocytes, and macrophages as a precursor. Pro-platelet basic protein or leukocyte derived grow factor (PPBP/LDGF) (a protein of ~14 kD) is modified by post-transcriptional cleavage to give rise to connective tissue activating peptide (CTAP-III), CXCL7, β -thromboglobulin (β-TG), and two variants of thrombocidin (TC-1 and TC-2). CTAP-III is activated by enzymatic proteolysis performed by cell surface-bound cathepsin G (CathG), a chymotryptic serine protease found in primary neutrophil granules. In this way, neutrophils are the mayor cells that convert CTAP-III into CXCL7. In addition, monocytes and mast cells can proteolytically modify CTAP-III to produce CXCL7. In fact, CathG has been detected in mast cells in addition to chymase, another chymotryptic enzyme. Further C-terminal truncations, that eliminate the last four and seven amino acids in CXCL7, generate two additional natural isoforms, these variants are more potent neutrophils activators. High levels of CTAP-III have been detected in plasma of patients with lung cancer. In fact, it has been suggested as a biomarker for early lung cancer detection. An increase in positive immunostained cells has been detected in the bronchial submucosa of patients with stable severe chronic obstructive pulmonary disease. Also, studies with premalignant breast cancer cells transfected with CXCL7 showed that these cells became as invasive as malignant breast cancer cells. In addition, CXCL7 induces stimulation of the lymphangiogenic factors VEGF-C and VEGF-D in human breast cancer cells, suggesting an important role for CXCL7 in tumor invasion.
- 分子量
- The 62 amino acid recombinant protein has a predicted molecular mass of approximately 6.7 kDa. The DTT-reduced and non-reduced protein migrate at approximately 11 and 9 kDa by SDS-PAGE respectively. The N-terminal amino acid is Ile.
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