TFPI Protein (His tag)
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- 抗原 See all TFPI 蛋白
- TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This TFPI protein is labelled with His tag.
- 应用范围
- Western Blotting (WB)
- 产品特性
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Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK(Dnp) - NH2. The IC50 value is < 0. 35 nM, as measured in 100 μL reaction mixture containing 1. 25 ng trypsin, 10μM substrate, 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0. 05 % Brij-35 (w/v), pH 7. 5.
- 纯度
- >90 % as determined by SDS-PAGE.
- Top Product
- Discover our top product TFPI 蛋白
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- 应用备注
- This recombinant protein can be used for WB. For research use only.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 储存条件
- -80 °C,-20 °C
- 储存方法
- Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
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- 抗原
- TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))
- 别名
- TFPI (TFPI 产品)
- 别名
- EPI Protein, LACI Protein, TFI Protein, TFPI1 Protein, A630013F22Rik Protein, AW552122 Protein, TFPI Protein, si:dz44f8.1 Protein, tfpib Protein, wu:fb92g01 Protein, zgc:152844 Protein, zgc:158592 Protein, tissue factor pathway inhibitor Protein, tissue factor pathway inhibitor a Protein, TFPI Protein, Tfpi Protein, tfpia Protein
- 背景
- Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF), The second K domain inhibits factor Xa, The third K domain binds to heparin, The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.
- 分子量
- 30 kDa
- 基因ID
- 7035
- NCBI登录号
- NP_006278
- UniProt
- P10646
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