HIV-1 p17 蛋白
HIV-1 p17
宿主: Human Immunodeficiency Virus (HIV)
宿主: 大肠杆菌（E. Coli）
Recombinant
> 90 % by SDS-PAGE (CBB staining)
ELISA, WB, DB, SDS
(3 references)-
- 抗原 See all HIV-1 p17 products
- HIV-1 p17 (Human Immunodeficiency Virus 1 Matrix (HIV-1 p17))
- 蛋白类型
- Recombinant
- 宿主
- Human Immunodeficiency Virus (HIV)
- 资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Western Blotting (WB), Dot Blot (DB), SDS-PAGE (SDS)
- 纯度
- > 90 % by SDS-PAGE (CBB staining)
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- 应用备注
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1) It can be used as p17 antigen in detection of anti-HIV-1 p17 antibody in Western blotting or ELISA.
2) It can be used as a standard for quantitative analysis of HIV-1 p17 antigen.
3) It can be used in studies of structure and function of AIDS virus as a matrix protein that constitutes HIV-1 core. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.23 mg/mL
- 缓冲液
- 50 % glycerol, 20 mM Tris-HCl ( pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol
- 储存条件
- -20 °C
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: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).
: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).
: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).
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: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).
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- 抗原
- HIV-1 p17 (Human Immunodeficiency Virus 1 Matrix (HIV-1 p17))
- 别名
- HIV-1 Gag p17 (HIV-1 p17 产品)
- 物质类
- Viral Protein
- 背景
- HIV-1 Gag p17 is the matrix protein of AIDS virus, HIV-1, and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable for reproduction of AIDS virus and is essential component of AIDS virus particles. This protein was over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p17 coding region of HIV-1 virus, subtype B, and was highly purified by several steps of chromatography. Its molecular weight is 17 kD, same as that of p17 purified from AIDS virus particles.
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