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Streptavidin Protein (FITC)

宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native FACS
产品编号 ABIN2145454
发货至: 中国
  • 抗原 See all Streptavidin 蛋白
    Streptavidin
    蛋白类型
    Native
    宿主
    • 40
    • 3
    • 1
    • 1
    • 1
    Streptomyces avidinii
    资源
    • 10
    • 8
    • 2
    • 1
    Streptomyces avidinii
    标记
    This Streptavidin protein is labelled with FITC.
    应用范围
    Flow Cytometry (FACS)
    产品特性
    The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity. Streptavidin-FITC has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.
    Top Product
    Discover our top product Streptavidin 蛋白
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add biotinylated primary antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 10 μL Streptavidin-FITC mixing with cells, incubate for 20 minutes at room temperature away from light,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    0.01M PBS, pH 7.4, 0.2 % (w/v) BSA
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • 抗原
    Streptavidin
    Abstract
    Streptavidin 产品
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