TEV Protease 蛋白
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for TEV Protease 蛋白 (ABIN2018393)
抗原
TEV Protease
蛋白类型
Recombinant
宿主
Tobacco Etch Virus (TEV)
资源
大肠杆菌（E. Coli）
纯度
> 95 % by SDS-PAGE analyses.
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产品特性
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6 IU/μl
Unit Definition: One unit of TEV protease cleaves > 85 % of 3 μg of control substrate in 1 hour at pH 8.0 at 30 °C. -
过滤
- Sterile
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Sterile liquid solution contains 50 mM Tris, 5 mM DTT, 50 % glycerol, pH 7.5.
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注意事项
- Avoid freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20 °C from date of receipt.
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有效期
- 12 months
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- TEV Protease
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物质类
- Viral Protein
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背景
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Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30 °C, also it can be used at temperature as low as 4 °C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques.
Synonyms: rTEV, TEV, P1 protease -
分子量
- 28.4 kDa, observed by reducing SDS-PAGE.
抗原
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