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KIT Ligand Protein (KITLG)

KITLG 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant Greater than 95.0 % as determined by the following methods:(a) Analysis by SEC-HPLC(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
产品编号 ABIN2018375
发货至: 中国
  • 抗原 See all KIT Ligand (KITLG) 蛋白
    KIT Ligand (KITLG)
    蛋白类型
    Recombinant
    生物活性
    Active
    宿主
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    资源
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    大肠杆菌(E. Coli)
    产品特性
    The ED50, as determined by the dose-dependant stimulation of human TF-1 cells, is < 2 ng/mL, corresponding to a specific activity of 5x10^5 IU/mg.
    The sequence of the first five N-terminal amino acids has been found to be Met-Glu-Gly-Ile-Cys.
    纯度
    Greater than 95.0 % as determined by the following methods:(a) Analysis by SEC-HPLC(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
    内毒素水平
    < 0.1 ng/μg (IEU/μg) of rHuSCF
    Top Product
    Discover our top product KITLG 蛋白
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    It is recommended that the lyophilized recombinant human stem cell factor (SCF) be reconstituted in sterile 18 M omega-cm H2O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.
    缓冲液
    The protein was lyophilized after extensive dialysis against 20 mM HAc-NaAc, pH 4.5, 200 mM NaCl buffer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    Lyophilized recombinant human stem cell factor (SCF) remains stable at room temperature for three weeks, but it is best stored desiccated below -18 °C. Upon reconstitution Recombinant Human Stem Cell Factor (SCF) should be stored at 4 °C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added.
  • Herraiz, Journé, Abdel-Malek, Ghanem, Jiménez-Cervantes, García-Borrón: "Signaling from the human melanocortin 1 receptor to ERK1 and ERK2 mitogen-activated protein kinases involves transactivation of cKIT." in: Molecular endocrinology (Baltimore, Md.), Vol. 25, Issue 1, pp. 138-56, (2011) (PubMed).

    Woost, Solchaga, Meyerson, Shankey, Goolsby, Jacobberger: "High-resolution kinetics of cytokine signaling in human CD34/CD117-positive cells in unfractionated bone marrow." in: Blood, Vol. 117, Issue 15, pp. e131-41, (2011) (PubMed).

  • 抗原
    KIT Ligand (KITLG)
    别名
    Stem Cell Factor (SCF) (KITLG 产品)
    别名
    Xkl-1 Protein, Xsl Protein, Xsl-1 Protein, Xsl-2 Protein, steel Protein, KITLG Protein, SCF Protein, kitl Protein, kl-1 Protein, mgf Protein, scf Protein, FPH2 Protein, KL-1 Protein, Kitl Protein, MGF Protein, SF Protein, SHEP7 Protein, Clo Protein, Con Protein, Gb Protein, Kitlg Protein, Mgf Protein, SLF Protein, Sl Protein, blz Protein, contrasted Protein, 710-712 Protein, CSF Protein, KITL Protein, KIT ligand L homeolog Protein, KIT ligand Protein, kit ligand Protein, kitlg.L Protein, KITLG Protein, kitlg Protein, Kitl Protein, Kitlg Protein
    背景
    Human Stem Cell Factor (SCF), produced in E. coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18,409 Da. Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. Stem Cell Factor (SCF) stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
    Synonyms: rHuSCF, SCF
    分子量
    18,409 Da
    途径
    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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