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Growth Hormone 1 Protein (GH1) (His tag)

Name: Growth Hormone 1 Protein (GH1) (His tag)
Brand: antibodies-online
SKU: ABIN1719793
Availability: OutOfStock

GH1 宿主: 人宿主: Tobacco (Nicotiana benthamiana) Recombinant > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. WB, CC Active

SDS

The biological activity of human Growth Hormone is measured by cell proliferation using Nb2-11 cells.

Cell migration after 24h of treatment with different concentrations of rh GH, scale bar 100 px, A: Untreated cells, B: Positive control Growth factor cocktail 10 %, C: 1 ng/mL rH GH, D: 5 ng/mL rH GH.

3 images

(1 reference)

产品编号 ABIN1719793

发货至: 中国

Datasheet as PDF

抗原 See all Growth Hormone 1 (GH1) 蛋白

Growth Hormone 1 (GH1)

蛋白类型

Recombinant

生物活性

Active
宿主
16

6

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资源
67

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Tobacco (Nicotiana benthamiana)

标记

This Growth Hormone 1 protein is labelled with His tag.

应用范围

Western Blotting (WB), Cell Culture (CC)

序列

HHHHHHFPTI PLSRPFDNAM LRAHRLHQLA FDTYQEFEEA YIPKEQKYSF LQNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGFAG

产品特性

Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody HGH. (Fig.1 Lanes 4-5).
Molecular formula: C1025H1570N280O306S7.
Isoelectric Point: 5,98.
Extinction coefficient: E 0.1 % (1g/L) = 0.77 (A 280 nm).
This product contains no animal-derived components or impurities. Produced by transient expression of human Growth Hormone in non-transgenic plants.

纯化方法

Recombinant human Growth Hormone contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).

纯度

> 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

内毒素水平

< 0.04 EU/µg protein (LAL method)
Growth Hormone 1 (GH1) (Active) protein
GH1 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active

Growth Hormone 1 (GH1) (AA 27-217) (Active) protein
GH1 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active

Growth Hormone 1 (GH1) protein
GH1 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE

Growth Hormone 1 (GH1) (AA 1-217) protein (GST tag)
GH1 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AA, AP

Growth Hormone 1 (GH1) (AA 27-217) protein (His tag)
Crystallography grade GH1 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys

Growth Hormone 1 (GH1) (AA 27-216) protein (His tag)
Crystallography grade GH1 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys

Growth Hormone 1 (GH1) protein
GH1 宿主: 小鼠 Recombinant BCA

Growth Hormone 1 (GH1) (AA 27-217) (Active) protein
GH1 宿主: 人宿主: CHO Cells Recombinant SDS, Func Active

Growth Hormone 1 (GH1) (Active) protein
GH1 宿主: 斑马鱼宿主: 大肠杆菌（E. Coli） Recombinant Func Active

Growth Hormone 1 (GH1) (Active) protein
GH1 宿主: Red Sea Bream 宿主: 大肠杆菌（E. Coli） Recombinant Func Active

说明

Biological Activity: The biological activity of human Growth Hormone is measured by cell proliferation using Nb2-11 cells. ED50 ≤ 0.04-0.1 ng/mL

限制

仅限研究用
状态

Lyophilized

溶解方式

Lyophilized protein should be reconstituted in water to a concentration of 200 ng/µL.

缓冲液

PBS 0.05 M buffer at pH 7.5

储存条件

4 °C
Bults, Sonesson, Knutsson, Bischoff, van de Merbel: "Intact protein quantification in biological samples by liquid chromatography - high-resolution mass spectrometry: somatropin in rat plasma." in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, Vol. 1144, pp. 122079, (2020) (PubMed).
抗原

Growth Hormone 1 (GH1)

别名

GH1 (GH1 产品)

别名

GH Protein, GH-N Protein, GHN Protein, IGHD1B Protein, hGH-N Protein, Gh1 Protein, GH1 Protein, gh Protein, Gh Protein, GH2 Protein, RNGHGP Protein, ghl Protein, gh-n Protein, ghn Protein, ighd1b Protein, gh1 Protein, ghb-A Protein, GHI Protein, GHB3 Protein, growth hormone 1 Protein, growth hormone Protein, somatotropin-like Protein, somatotropin Protein, growth hormone prepeptide Protein, growth hormone 1 L homeolog Protein, growth hormone 1 S homeolog Protein, Somatotropin-1 Protein, GH1 Protein, Gh Protein, GH Protein, LOC100305005 Protein, Gh1 Protein, LOC100534452 Protein, LOC100232594 Protein, LOC100303681 Protein, gh1 Protein, LOC100356068 Protein, LOC100136588 Protein, gh1.L Protein, gh1.S Protein, LOC109081196 Protein

背景

Synonyms: GH, GH-N, GHN, IGHD1B, hGH-N, pituitary growth hormone, somatotropin
GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation, an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.

分子量

22.9 kDa

基因ID

2688, 139250

途径

NF-kappaB Signaling, JAK/STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process