Recombinant Human Protein Disulfide-Isomerase A4/PDIA4 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Val21-Leu645) of Human PDIA4 fused with a polyhistidine tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
PDIA4
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Liquid
溶解方式
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,10 % Glycerol, pH 7.5.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
-80 °C
储存方法
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
有效期
6 months
抗原
PDIA4
(Protein Disulfide Isomerase Family A, Member 4 (PDIA4))
LOC100229535 Protein, cai Protein, wu:fd20c07 Protein, zgc:113965 Protein, zgc:136625 Protein, zgc:56014 Protein, zgc:77773 Protein, AI987846 Protein, Cai Protein, ERp-72 Protein, Erp72 Protein, ERP70 Protein, ERP72 Protein, Erp70 Protein, protein disulfide isomerase family A member 4 Protein, protein disulfide isomerase family A, member 4 Protein, protein disulfide isomerase associated 4 Protein, protein disulfide isomerase family A member 4 L homeolog Protein, PDIA4 Protein, pdia4 Protein, Pdia4 Protein, pdia4.L Protein
Sub Type
Fusionprotein
背景
Protein Disulfide-Isomerase A4 (PDIA4) is an endoplasmic reticulum luminal protein that belongs to the protein disulfide isomerase family. Human PDIA4 is synthesized as a 625 amino acid precursor that contains a 20 amino acid signal sequence, and a 625 amino acid mature chain, including three thioredoxin domains. PDIA4 catalyzes the rearrangement of -S-S- bonds in proteins and is thought to be a deoxycytidine kinase. In addition, PDIA4 serves as a proteases protein disulfide isomerase, phospholipase or an arrangement of these. Alternative Names: Protein Disulfide-Isomerase A4, Endoplasmic Reticulum Resident Protein 70, ER Protein 70, ERp70, Endoplasmic Reticulum Resident Protein 72, ER Protein 72, ERp-72, ERp72, PDIA4, ERP70, ERP72