GRP78 Protein (full length)
(4 references)-
- 抗原 See all GRP78 (HSPA5) 蛋白
- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
- 蛋白类型
- Recombinant
- 产品特性
- full length
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Functional Studies (Func), Activity Assay (AcA)
- 序列
- MKLSLVAAML LLLSAARAEE EDKKEDVGTV VGIDLGTTYS CVGVFKNGRV EIIANDQGNR ITPSYVAFTP EGERLIGDAA KNQLTSNPEN TVFDAKRLIG RTWNDPSVQQ DIKFLPFKVV EKKTKPYIQV DIGGGQTKTF APEEISAMVL TKMKETAEAY LGKKVTHAVV TVPAYFNDAQ RQATKDAGTI AGLNVMRIIN EPTAAAIAYG LDKREGEKNI LVFDLGGGTF DVSLLTIDNG VFEVVATNGD THLGGEDFDQ RVMEHFIKLY KKKTGKDVRK DNRAVQKLRR EVEKAKRALS SQHQARIEIE SFYEGEDFSE TLTRAKFEEL NMDLFRSTMK PVQKVLEDSD LKKSDIDEIV LVGGSTRIPK IQQLVKEFFN GKEPSRGINP DEAVAYGAAV QAGVLSGDQD TGDLVLLDVC PLTLGIETVG GVMTKLIPRN TVVPTKKSQI FSTASDNQPT VTIKVYEGER PLTKDNHLLG TFDLTGIPPA PRGVPQIEVT FEIDVNGILR VTAEDKGTGN KNKITITNDQ NRLTPEEIER MVNDAEKFAE EDKKLKERID TRNELESYAY SLKNQIGDKE KLGGKLSSED KETMEKAVEE KIEWLESHQD ADIEDFKAKK KELEEIVQPI ISKLYGSAGP PPTGEEDTAE KDEL
- 特异性
- ~78 kDa
- 纯化方法
- Affinity Purified
- 纯度
- >90%
- Top Product
- Discover our top product HSPA5 蛋白
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Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 19-654) protein (GST tag)
HSPA5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 20-650) protein (His tag)HSPA5 宿主: 人 宿主: Hi-5 Cells Recombinant > 90 % by SDS - PAGE SDS
Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 20-650) protein (His tag)HSPA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 269-354) protein (GST tag)HSPA5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 19-654) protein (His tag)HSPA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 20-655) protein (His tag)Crystallography grade HSPA5 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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This product has been certified >90% pure using SDS-PAGE analysis and gamma globulin as the protein concentration standard.
- 限制
- 仅限研究用
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- 浓度
- Lot specific
- 缓冲液
- 20 mM Tris/HCl pH 7.5, 0.45M NaCl, 10 % glycerol, 0.5 mM DTT
- 储存条件
- -20 °C
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: "HKH40A downregulates GRP78/BiP expression in cancer cells." in: Cell death & disease, Vol. 5, pp. e1240, (2014) (PubMed).
: "Bcl-2 associated athanogene 5 (Bag5) is overexpressed in prostate cancer and inhibits ER-stress induced apoptosis." in: BMC cancer, Vol. 13, pp. 96, (2013) (PubMed).
: "IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 4, pp. 1614-24, (2013) (PubMed).
: "A peptidic unconjugated GRP78/BiP ligand modulates the unfolded protein response and induces prostate cancer cell death." in: PLoS ONE, Vol. 7, Issue 10, pp. e45690, (2012) (PubMed).
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: "HKH40A downregulates GRP78/BiP expression in cancer cells." in: Cell death & disease, Vol. 5, pp. e1240, (2014) (PubMed).
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- 抗原
- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
- 别名
- Grp78 (Bip) (HSPA5 产品)
- 别名
- GRP78 Protein, BiP Protein, GRP-78 Protein, grp78 Protein, hspa5a Protein, BIP Protein, MIF2 Protein, AL022860 Protein, AU019543 Protein, Bip Protein, D2Wsu141e Protein, D2Wsu17e Protein, Grp78 Protein, Hsce70 Protein, SEZ-7 Protein, Sez7 Protein, baffled Protein, mBiP Protein, cb865 Protein, fb60h09 Protein, fi36d04 Protein, wu:fb60h09 Protein, wu:fi36d04 Protein, zgc:55994 Protein, zgc:77606 Protein, 78 kDa glucose-regulated protein Protein, heat shock protein family A (Hsp70) member 5 Protein, BiP/GRP78 Protein, glucose-regulated protein 78 Protein, putative glucose-regulated protein 78 Protein, Hsp70 family ATPase KAR2 Protein, heat shock protein family A (Hsp70) member 5 S homeolog Protein, heat shock 70 kDa protein 5a Protein, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) Protein, heat shock protein 5 Protein, heat shock protein family A member 5 Protein, CpipJ_CPIJ003550 Protein, HSPA5 Protein, grp78 Protein, LOC100533358 Protein, BiP/grp78 Protein, Tc00.1047053506585.40 Protein, Tb11.02.5450 Protein, Tb11.02.5500 Protein, LMJF_28_1200 Protein, KAR2 Protein, LOC100135840 Protein, hspa5.S Protein, hspa5 Protein, Hspa5 Protein
- 背景
- GRP78 is a ubiquitously expressed, 78- kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia (Barnes), to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
- 分子量
- approx. 78 kDa
- 基因ID
- 3309
- NCBI登录号
- NM_005347
- UniProt
- P11021
- 途径
- Thyroid Hormone Synthesis, ER-Nucleus Signaling
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