(2 references)-
- 抗原 See all CCL2 蛋白
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Functional Studies (Func)
- 品牌
- BD Pharmingen™
- 特异性
- Bioactivity: This material has been reported to have bioactivity when measured by Ca 2+ Flux Assay, using Human THP-1cells. The EC50 is commonly in the range of 20-350 ng/ml. Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
- 产品特性
- Recombinant human MCP-1 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution (pH 7.2) and containing 2 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The human MCP-1 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
- 纯化方法
- Recombinant human MCP-1 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution and bovine serum albumin, with no preservatives. Recombinant human MCP-1 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
- 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng per µg of human MCP-1, as measured in a chromogenic LAL assay.
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- Discover our top product CCL2 蛋白
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Chemokine (C-C Motif) Ligand 2 (CCL2) protein (His-Avi Tag,Biotin)
CCL2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Chemokine (C-C Motif) Ligand 2 (CCL2) protein (His-Avi Tag)CCL2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-99) protein (His tag)CCL2 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chemokine (C-C Motif) Ligand 2 (CCL2) (Active) proteinCCL2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-99) (Active) protein (Biotin)CCL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE CMA, FACS Active
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-99) (Active) proteinCCL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE CMA Active
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 1-99) protein (GST tag)CCL2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-99) protein (GST tag)CCL2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-C Motif) Ligand 2 (CCL2) protein (His tag)CCL2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 1-290) protein (His tag)Crystallography grade CCL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- ELISA Standard: The human MCP-1 (Cat. No. 554620) is useful as a quantitative standard for measuring human MCP-1 protein levels in a sandwich ELISA with the purified 10F7 antibody (Cat. No. 555055) as a capture antibody and the biotinylated 5D3-F7 (Cat. No. 554664) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human MCP-1 standard from 2000-4 pg/ml should be included in each ELISA plate.
- 说明
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BD Pharmingen™ Recombinant Human MCP-1
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- Frozen aqueous buffered solution containing BSA.
- 储存液
- Azide free
- 注意事项
- Human monocyte chemoattractant protein-1 (MCP-1), also known as MCAF (monocyte chemotactic and activating factor), is a member of CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells.
- 储存条件
- -80 °C
- 储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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- 抗原
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- 别名
- MCP-1 (CCL2 产品)
- 别名
- GDCF-2 Protein, HC11 Protein, HSMCR30 Protein, MCAF Protein, MCP-1 Protein, MCP1 Protein, SCYA2 Protein, SMC-CF Protein, AI323594 Protein, JE Protein, Scya2 Protein, Sigje Protein, MCP-1A Protein, MCP1A Protein, C-C motif chemokine ligand 2 Protein, chemokine (C-C motif) ligand 2 Protein, C-C motif chemokine 2 Protein, CCL2 Protein, Ccl2 Protein, LOC101120093 Protein
- 背景
- Human monocyte chemoattractant protein-1 (MCP-1), also known as MCAF (monocyte chemotactic and activating factor), is a member of CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells.
- 途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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