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- 抗原 See all HMGB1 蛋白
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人, 小鼠, 大鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Activation (Act), Cellular Assay (CA)
- 序列
- MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE
- 产品特性
- The product can be used in cell migration assays, both in vitro and in vivo, maximum activity is at 1 nM. Intraperitoneal injection in the mouse recruits neutrophils, monocytes and macrophages. Injection of 2.5 µg HMGB1 in the mouse hippocampus increases epileptic activity after kainate injection (Maroso et al, Nature Medicine 2010, 16: 413-9). Chemotaxis-HMGB1 is a 25 kDa nuclear protein, present in almost all mammalian cells. The protein is almost identical (213/215 aa) in human, mouse, rat. This product corresponds to the rat sequence and is produced in E.coli. It contains only trace amounts of LPS (<0.4 ng/mg protein), and is tested for the ability to induce fibroblast migration. This formulation is fully reduced and DOES NOT induce cytokine/chemokine secretion when given to target cells. LPS-free. Measured by its ability to induce migration. Maximal activity in the cell migration assay is obtained at 1 nM, or 25 ng/ml. The Chemotaxis-HMGB1 we provide is the natural protein, with no tags or additional amino acids. It is produced in E.coli from an expression plasmid coding for the rat protein.
- 纯化方法
- Purified
- 纯度
- > 95 %
- 内毒素水平
- The purified protein is free from LPS (Cambrex Limulus Amoebocyte Assay QCL-1000, <0.4 ng LPS per mg protein).
- Top Product
- Discover our top product HMGB1 蛋白
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High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag,Biotin)HMGB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
High Mobility Group Box 1 (HMGB1) (Non-oxidizable) (Active) proteinHMGB1 宿主: 人, 小鼠, 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 宿主: 人 宿主: Hi-5 Cells Recombinant > 90 % by SDS - PAGE SDS
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
High Mobility Group Box 1 (HMGB1) (AA 2-215) protein (His tag)Crystallography grade HMGB1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
High Mobility Group Box 1 (HMGB1) (AA 2-215) protein (His tag)Crystallography grade HMGB1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 50 mM HEPES-Na pH 7.9, 500 mM NaCl, 0.5 mM DTT.
- 注意事项
- Oxidation of cysteine 106 makes the protein inactive. To avoid cysteine oxidation, DTT 0.5 mM is added during protein purification.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The protein once resuspended can be stored frozen (-20°C), thawed and re-frozen.
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- 抗原
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- 别名
- High-mobility group protein B1 (HMGB1) (HMGB1 产品)
- 别名
- HMG1 Protein, HMG3 Protein, SBP-1 Protein, DEF Protein, HMG-1 Protein, Hmg1 Protein, amphoterin Protein, p30 Protein, hmgb1 Protein, ik:tdsubc_1a5 Protein, wu:fb23c02 Protein, xx:tdsubc_1a5 Protein, zgc:56110 Protein, zgc:77104 Protein, hmg-1 Protein, hmg3 Protein, sbp-1 Protein, hmg1 Protein, HMGB1 Protein, Ac2-008 Protein, high mobility group box 1 Protein, high-mobility group box 1 Protein, high mobility group box 1a Protein, high mobility group box 1 L homeolog Protein, high mobility group protein B1 Protein, HMGB1 Protein, Hmgb1 Protein, hmgb1 Protein, hmgb1a Protein, hmgb1.L Protein, LOC100359149 Protein
- 背景
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HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells. HMGB1 is essential for life: Hmgb1 knockout mice die shortly after birth. Just recently, the two different activities of HMGB1 as a chemoattractanct of motile cells and as inducer of cytokines have been attributed to different biochemical forms. Chemotaxis-HMGB1 (the form with chemoattractanct activity) is completely reduced, cytokine-HMGB1 contains a disulfide bond between C23 and C45.Chemotaxis-HMGB1 had previously been sold by HMGBiotech as HMGB1 LPS free and tested in migration assay, but is otherwise the same identical product.Chemotaxis-HMGB1 behaves as a trigger of inflammation, attracting inflammatory cells, and of tissue repair, attracting stem cells and promoting their proliferation. Moreover, Chemotaxis-HMGB1 activates dedritic cells and promotes their functional maturation and migration to lymph nodes.However, and contrary to early reports, pure Chemotaxis-HMGB1 does not promote cytokine secretion, it does so only when it interacts with other molecules, like LPS, single-stranded DNA, or IL-1beta. High Mobility Group Box 1 Protein Binding to Lipopolysaccharide Facilitates Transfer of Lipopolysaccharide to CD14 and Enhances Lipopolysaccharide-Mediated TNF-alpha Production in Human Monocytes.
HMGB1 consists of two fairly rigid, L-shaped DNA-binding domains, each referred to as a ‘HMG box’, and an unstructured tail that ends with 30 consecutive negatively charged amino acids. HMGB1 consists of 215 amino acid residues and has a calculated molecular mass of approximately 24.8 kDa. It migrates at a position of approximately 30 kD in SDS-PAGE gels, possibly because of the unusual number of positively charged amino acids it contains. - 分子量
- 25 kDa
- 途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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