NCF4 Protein (His tag)
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- 抗原 See all NCF4 蛋白
- NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 标记
- This NCF4 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by chromatography
- 纯度
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product NCF4 蛋白
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- 说明
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Synonyms: Neutrophil cytosol factor 4, NCF, P40PHOX, SH3PXD4
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM DTT
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
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- 抗原
- NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
- 别名
- NCF4 (NCF4 产品)
- 别名
- p40phox Protein, AI451400 Protein, NCF Protein, P40PHOX Protein, SH3PXD4 Protein, neutrophil cytosolic factor 4 Protein, ncf4 Protein, Ncf4 Protein, NCF4 Protein
- 背景
- NCF4 is a cytosolic regulatory of the superoxide-producing phagocyte NADPH-oxidase, amulticomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integratedcatalytic core of the enzyme system. Recombinant human NCF4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 41.1 kDa (359aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_000622
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