IFI30 Protein (His tag)
-
- 抗原 See all IFI30 蛋白
- IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))
- 蛋白类型
- Recombinant
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This IFI30 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by chromatography
- 纯度
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product IFI30 蛋白
-
Interferon, gamma-Inducible Protein 30 (IFI30) (AA 58-232) protein (His tag)
IFI30 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interferon, gamma-Inducible Protein 30 (IFI30) (AA 1-250) protein (GST tag)IFI30 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interferon, gamma-Inducible Protein 30 (IFI30) (AA 58-232) protein (His tag)Crystallography grade IFI30 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interferon, gamma-Inducible Protein 30 (IFI30) (AA 55-230) protein (His tag)Crystallography grade IFI30 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interferon, gamma-Inducible Protein 30 (IFI30) protein (Myc-DYKDDDDK Tag)IFI30 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 说明
-
Synonyms: Gamma-interferon-inducible lysosomal thiol reductase, GILT, IFI-30, IP30
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM DTT
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
-
- 抗原
- IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))
- 别名
- IFI30 (IFI30 产品)
- 别名
- GILT Protein, wu:fb12b04 Protein, zgc:101752 Protein, IP30 Protein, IFI-30 Protein, interferon, gamma-inducible protein 30 Protein, IFI30, lysosomal thiol reductase Protein, interferon gamma inducible protein 30 Protein, ifi30 Protein, IFI30 Protein, LOC100304719 Protein, Ifi30 Protein
- 背景
- IFI30, also as known as gamma-interferon-inducible lysosomal thiol reductase, belongs to the GILT family. This protein is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. This protein facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. Recombinant human IFI30 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
- 分子量
- 22.5 kDa (199aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_006323
-
