Kininogen Protein (KNG) (AA 19-427) (His tag)
KNG
宿主: 人
宿主: 人细胞
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- 抗原 See all Kininogen (KNG) products
- Kininogen (KNG)
- 蛋白类型
- Recombinant
- 产品特性
- AA 19-427
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宿主
- 人
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资源
- 人细胞
- 标记
- This Kininogen protein is labelled with His tag.
- 原理
- Recombinant Human Kininogen-1/KNG1 (C-6His)
- 序列
- QESQSEEIDC NDKDLFKAVD AALKKYNSQN QSNNQFVLYR ITEATKTVGS DTFYSFKYEI KEGDCPVQSG KTWQDCEYKD AAKAATGECT ATVGKRSSTK FSVATQTCQI TPAEGPVVTA QYDCLGCVHP ISTQSPDLEP ILRHGIQYFN NNTQHSSLFM LNEVKRAQRQ VVAGLNFRMT YSIVQTNCSK ENFLFLTPDC KSLWNGDTGE CTDNAYIDIQ LRIASFSQNC DIYPGKDFVQ PPTKICVGCP RDIPTNSPEL EETLTHTITK LNAENNATFY FKIDNVKKAR VQVVAGKKYF IDFVARETTC SKESNEELTE SCETKKLGQS LDCNAEVYVV PWEKKIYPTV NCQPLGMISL MKRPPGFSPF RSSRIGEIKE ETTSHLRSCE YKGRPPKAGA EPASEREVSL DHHHHHH
- 产品特性
- Recombinant Human Kininogen-1/KNG1 produced by transfected human cells is a secreted protein with sequence (Gln19-Ser427) of human KNG1 fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM Hac-NaAC, 150 mM NaCl, pH 4.0.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- Kininogen (KNG)
- 别名
- kininogen (KNG 产品)
- 别名
- kininogen Protein, kininogen 1 Protein, Kng1 Protein
- Sub Type
- Fusionprotein
- 背景
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Kininogen-1 is a secreted protein which contains three cystatin domains. There are two alternatively spliced forms, designated as the high molecular weight (HMW) and low MW (LMW) forms. Kininogen-1 plays a critical role in blood coagulation and inflammatory response. Kininogens are inhibitors of thiol proteases. Kininogen-1 participates in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII, also inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. The active peptide bradykinin that is released from Kininogen-1 shows a variety of physiological effects: influence in smooth muscle contraction, induction of hypotension, natriuresis and diuresis, decrease in blood glucose level. It is a mediator of inflammation and causes increase in vascular permeability, stimulation of nociceptors release of other mediators of inflammation. It has a cardioprotective effect. LMW-kininogen inhibits the aggregation of thrombocytes and doesn’t involved in blood clotting.
Alternative Names: Kininogen-1, lpha-2-Thiol Proteinase Inhibitor, Fitzgerald Factor, High Molecular Weight Kininogen, HMWK, Williams-Fitzgerald-Flaujeac Factor, KNG1, BDK, KNG - 分子量
- 46.89 kDa
- UniProt
- P01042
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