Biglycan Protein (BGN) (AA 20-368) (His tag)
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- 抗原 See all Biglycan (BGN) 蛋白
- Biglycan (BGN)
- 蛋白类型
- Recombinant
- 产品特性
- AA 20-368
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宿主
- 人
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资源
- 人细胞
- 标记
- This Biglycan protein is labelled with His tag.
- 原理
- Recombinant Human Biglycan/BGN (C-6His)
- 序列
- EQRGFWDFTL DDGPFMMNDE EASGADTSGV LDPDSVTPTY SAMCPFGCHC HLRVVQCSDL GLKSVPKEIS PDTTLLDLQN NDISELRKDD FKGLQHLYAL VLVNNKISKI HEKAFSPLRK LQKLYISKNH LVEIPPNLPS SLVELRIHDN RIRKVPKGVF SGLRNMNCIE MGGNPLENSG FEPGAFDGLK LNYLRISEAK LTGIPKDLPE TLNELHLDHN KIQAIELEDL LRYSKLYRLG LGHNQIRMIE NGSLSFLPTL RELHLDNNKL ARVPSGLPDL KLLQVVYLHS NNITKVGVND FCPMGFGVKR AYYNGISLFN NPVPYWEVQP ATFRCVTDRL AIQFGNYKKV DHHHHHH
- 产品特性
- Recombinant Human Biglycan is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Glu20-Lys368) of Human BGN fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product BGN 蛋白
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Biglycan (BGN) (AA 1-368) protein (GST tag)
BGN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Biglycan (BGN) protein (Fc Tag)BGN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE
Biglycan (BGN) (AA 38-368) protein (His tag)Crystallography grade BGN 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Biglycan (BGN) (AA 38-369) protein (His tag)Crystallography grade BGN 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- Biglycan (BGN)
- 别名
- biglycan (BGN 产品)
- 别名
- DSPG1 Protein, PG-S1 Protein, PGI Protein, SLRR1A Protein, BG Protein, BSPG1 Protein, bgn Protein, zgc:91989 Protein, dspg1 Protein, pg-s1 Protein, slrr1a Protein, xbgn Protein, BGN Protein, zgc:77123 Protein, DKFZp468I2427 Protein, biglycan Protein, biglycan L homeolog Protein, biglycan a Protein, biglycan S homeolog Protein, biglycan b Protein, BGN Protein, Bgn Protein, bgn.L Protein, bgna Protein, bgn.S Protein, bgn Protein, bgnb Protein
- Sub Type
- Fusionprotein
- 背景
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Biglycan is a 200-350 kD proteoglycan consisting of a 45 kD core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains. Biglycan binds to TGF-?. It also binds to collagen type I in low ionic strength (less than 3 mM phosphate) buffer. At higher ionic strengths, Biglycan does not bind to collagen type I. It enhances the inhibition effect of TGF-? on osteoclast proliferation at a concentration of 4-20 mg/ml. It also prevents the attachment of CHO cells to fibronectin, with a 50% inhibition at 17-21 mg/ml.
Alternative Names: Biglycan, Bone/Cartilage Proteoglycan I, PG-S1, BGN, SLRR1A - 分子量
- 40.47 kDa
- UniProt
- P21810
- 途径
- Glycosaminoglycan Metabolic Process
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