30S Ribosomal Protein S13 (AA 2-118), (full length) protein (GST tag)
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- 抗原
- 30S Ribosomal Protein S13
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-118, full length
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宿主
- E. coli
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资源
- 大肠杆菌(E. Coli)
- 标记
- GST tag
- 应用范围
- ELISA, SDS-PAGE (SDS)
- 序列
- ARIAGINIPD HKHAVIALTS IYGVGKTRSK AILAAAGIAE DVKISELSEG QIDTLRDEVA KFVVEGDLRR EISMSIKRLM DLGCYRGLRH RRGLPVRGQR TKTNARTRKG PRKPIKK
- 产品特性
- Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. Ref.11 In the E.coli 70S ribosome in the initiation state (Ref.14) was modeled to contact the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits, bridge B1a is broken in the model with bound EF-G, while the protein-protein contacts between S13 and L5 in B1b change (Ref.14). The 23S rRNA contact site in bridge B1a is modeled to differ in different ribosomal states (Ref.15), contacting alternately S13 or S19. In the two 3.5 angstroms resolved ribosome structures (Ref.16) the contacts between L5, S13 and S19 bridge B1b are different, confirming the dynamic nature of this interaction. Bridge B1a is not visible in the crystallized ribosomes due to 23S rRNA disorder. Ref.11 Contacts the tRNAs in the A and P sites. Ref.11 The C-terminal tail plays a role in the affinity of the 30S P site for different tRNAs. Ref.11
- 纯度
- 90 %
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- 说明
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Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS buffer, 20mM GSH
- 储存条件
- -20 °C
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Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli." in: Nucleic acids research, Vol. 13, Issue 11, pp. 3891-903, (1985) (PubMed).
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Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli." in: Nucleic acids research, Vol. 13, Issue 11, pp. 3891-903, (1985) (PubMed).
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- 抗原
- 30S Ribosomal Protein S13
- 分子量
- 40.4 kD
- UniProt
- P0A7S9
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