anti-小鸡 Stratifin 抗体 for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Stratifin Antibody (supplied by: Log in to see )

抗原
Stratifin (SFN) 抗体
  • YWHAS
  • Er
  • Mme1
  • Ywhas
  • stratifin
  • SFN
  • Sfn
抗原表位
N-Term
28
22
21
13
10
10
10
7
7
6
4
3
3
3
3
3
2
1
1
1
1
1
1
适用
小鸡, 牛, 人, 小鼠, 兔, 大鼠, 绵羊
208
78
58
14
14
13
10
10
8
7
4
4
4
2
2
1
1
1
1
1
1
宿主

163
29
22
2
1
克隆类型
多克隆
标记
This Stratifin antibody is un-conjugated
13
10
7
6
5
5
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
188
86
83
48
48
24
22
18
13
8
3
3
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

产品编号 ABIN461974
$ 550.00
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1739741 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0

General

抗原 Stratifin (SFN) 抗体
抗原表位 N-Term
(28), (22), (21), (13), (10), (10), (10), (7), (7), (6), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
适用 小鸡, 牛, 人, 小鼠, 兔, 大鼠, 绵羊
(208), (78), (58), (14), (14), (13), (10), (10), (8), (7), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1)
宿主
(163), (29), (22), (2), (1)
克隆类型
多克隆
标记 This Stratifin antibody is un-conjugated
(13), (10), (7), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(188), (86), (83), (48), (48), (24), (22), (18), (13), (8), (3), (3), (2), (2), (1)
Supplier Log in to see

Stratifin抗体详情

Stratifin目标详情 使用细节 贮存及处理 图像
品牌 IHC-plus™
特异性 Recognizes 14-3-3 sigma in all mammals. It is a member of the 14-3-3 family which consists of 30 kD proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 sigma is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary. 14-3-3 sigma binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3 sigma inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3 sigma through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3 sigma targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1. May not react with recombinant proteins that are not N-acetylated.
免疫原 Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 sigma.

Type of Immunogen: Synthetic peptide
亚型 IgG
Plasmids, Primers & others

Stratifin目标详情

Stratifin抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 SFN / Stratifin / 14-3-3 Sigma (SFN Antibody 摘要)
背景 Name/Gene ID: SFN

Synonyms: SFN, 14-3-3 sigma, 14-3-3 protein sigma, HME1, Stratifin, YWHAS
基因ID 2810
UniProt P31947
途径 p53 Pathway, Myometrial Relaxation and Contraction

使用细节

Stratifin抗体详情 Stratifin目标详情 贮存及处理 图像 回页首
应用备注 Approved: IHC, IHC-P (1:250), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:250. Western Blot: LS-B3281 detects a band of approximately 30kD in HEK293 cell lysates.
说明

Target Species of Antibody: Sheep

限制 仅限研究用

贮存及处理

Stratifin抗体详情 Stratifin目标详情 使用细节 图像 回页首
状态 Liquid
浓度 Lot specific
缓冲液 Serum, 0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeat freeze-thaw cycles.
储存条件 4 °C,-20 °C
储存方法 Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

图像

Stratifin抗体详情 Stratifin目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Stratifin (SFN) (N-Term) antibody (ABIN461974) Human Skin (formalin-fixed, paraffin-embedded) stained with SFN antibody ABIN461974 a...
Immunohistochemistry (IHC) image for anti-Stratifin (SFN) (N-Term) antibody (ABIN461974) Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-...