anti-人 Tumor Necrosis Factor 抗体 for Neutralization

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

抗原
Tumor Necrosis Factor (TNF) 抗体
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LOC103694380
  • tnfa
适用

1380
530
459
229
223
203
202
59
56
44
32
18
15
9
7
6
6
5
4
4
4
4
3
2
2
1
1
1
1
宿主

1071
543
138
52
11
10
8
7
5
2
1
克隆类型
多克隆
标记
This Tumor Necrosis Factor antibody is un-conjugated
185
95
71
55
49
26
23
22
21
20
19
19
19
19
19
19
18
17
11
11
11
11
11
11
11
11
11
11
11
11
11
11
11
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
应用范围
Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
830
806
756
468
405
269
206
170
133
120
100
67
48
42
26
26
25
17
15
13
9
9
9
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
选项
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产品编号 ABIN5596796
$ 583.50
Plus shipping costs $45.00 and $23.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.755764 ABIN2749005 ICS Neut IHC ELISA Mouse IgG1 Log in to see B-C7 0
12.755764 ABIN2192175 IA FACS ISt Neut WB Rabbit Log in to see Polyclonal 0
7 ABIN465605 ELISA Neut Mouse IgG1 Log in to see 0
7 ABIN465030 ELISA Neut WB Rabbit Log in to see Polyclonal 0
7 ABIN465674 ELISA Neut WB Goat Log in to see Polyclonal 0
7 ABIN1104720 IHC IHC (p) Neut WB Rabbit Log in to see Polyclonal 0
5.5 ABIN1177286 Neut ELISA Mouse IgG2a, kappa Log in to see MABTNF-A5 3
5.5 ABIN1176946 Neut ELISA Mouse IgG1 Log in to see MAb1 2
4 ABIN222906 IHC Neut WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN2682656 Neut Rabbit IgG AA 77-233 Log in to see R101 0
4 ABIN5508607 Neut Mouse IgG1 AA 77-233 Log in to see MM0N1 0
1 ABIN199738 FACS Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6252283 Inhibition FACS IF Neut WB Mouse IgG1, kappa Log in to see J2D10 0
1 ABIN1959983 BR ELISA FACS Func ICC IHC IHC (fro) Neut Mouse IgG1 Log in to see MAb11 0
1 ABIN1959982 BR ELISA Func Neut WB Mouse IgG1 Log in to see MAb1 0
1 ABIN1967143 BR ELISA Func Neut WB Mouse IgG1 Log in to see MAb1 0
1 ABIN1449154 Neut Mouse IgG1 Log in to see #1 0
1 ABIN5526231 ELISA Neut HRP Mammalian Cells IgG4 kappa Log in to see CDP 571 0
1 ABIN465677 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN199734 ICC Neut ELISA WB Mouse IgG1 Log in to see 0

General

抗原 Tumor Necrosis Factor (TNF) 抗体
适用
(1380), (530), (459), (229), (223), (203), (202), (59), (56), (44), (32), (18), (15), (9), (7), (6), (6), (5), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1)
宿主
(1071), (543), (138), (52), (11), (10), (8), (7), (5), (2), (1)
克隆类型
多克隆
标记 This Tumor Necrosis Factor antibody is un-conjugated
(185), (95), (71), (55), (49), (26), (23), (22), (21), (20), (19), (19), (19), (19), (19), (19), (18), (17), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
应用范围 Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(830), (806), (756), (468), (405), (269), (206), (170), (133), (120), (100), (67), (48), (42), (26), (26), (25), (17), (15), (13), (9), (9), (9), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 找到1个引用
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Tumor Necrosis Factor抗体详情

Tumor Necrosis Factor目标详情 使用细节 贮存及处理 References for anti-Tumor Necrosis Factor antibody (ABIN5596796) 图像
特异性 This antibody is primarily directed against mature 17,000 MW human TNFalpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFalpha in the same formats using similar dilutions. The antibody does not recognize human TNGß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFalpha as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFalpha activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
产品特性 Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-alpha) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
过滤 Sterile filtered
免疫原 The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFalpha produced in E.coli.
Immunogen Type: RecombinantProtein
亚型 IgG
Plasmids, Primers & others

Tumor Necrosis Factor目标详情

Tumor Necrosis Factor抗体详情 使用细节 贮存及处理 References for anti-Tumor Necrosis Factor antibody (ABIN5596796) 图像 回页首
抗原
别名 TNF alpha (TNF Antibody 摘要)
背景 Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody
基因ID 7124
UniProt P01375
途径 NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

使用细节

Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 贮存及处理 References for anti-Tumor Necrosis Factor antibody (ABIN5596796) 图像 回页首
应用备注 This IgG fraction antibody of anti-Human TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown.
说明

Gene Name: TNFA

限制 仅限研究用

贮存及处理

Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 使用细节 References for anti-Tumor Necrosis Factor antibody (ABIN5596796) 图像 回页首
状态 Liquid
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Without preservative
储存条件 4 °C/-20 °C
储存方法 Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
有效期 12 months

References for anti-Tumor Necrosis Factor antibody (ABIN5596796)

Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Kant, Swat, Zhang, Zhang, Neel, Flavell, Davis: "TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway." in: Genes & development, Vol. 25, Issue 19, pp. 2069-78, 2011

图像

Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 使用细节 贮存及处理 References for anti-Tumor Necrosis Factor antibody (ABIN5596796) 回页首
厂商提供的图像
 image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) anti-Tumor Necrosis Factor (TNF) antibody
 image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)
Immunofluorescence (IF) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) Fluorescent immunohistochemistry showing staining of human colon by anti-TNF alpha (...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) Western blot of Anti-Human TNF-a (RABBIT) Antibody. Lane 1: 250ng human recombinant T...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) Western Blot of Rabbit anti-TNF Alpha antibody. Marker: Opal Pre-stained ladder . Lan...
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) Western blot using Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TB...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN5596796) Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA...