anti-小鼠 TNFRSF11A 抗体 for Flow Cytometry

Recommended TNFRSF11A Antibody (supplied by: Log in to see )

抗原
Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) 抗体
  • RANK
  • RGD1563614
  • TNFRSF11A
  • Ly109
  • ODFR
  • OFE
  • Rank
  • TRANCE-R
  • mRANK
  • CD265
  • FEO
  • LOH18CR1
  • OPTB7
  • OSTS
  • PDB2
  • TRANCER
  • TNF receptor superfamily member 11A
  • TNF receptor superfamily member 11a
  • tumor necrosis factor receptor superfamily, member 11a, NFKB activator
  • Tnfrsf11a
  • TNFRSF11A
抗原表位
AA 326-616, AA 330-427
20
20
16
15
10
4
4
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
适用
人, 小鼠, 大鼠
143
76
51
宿主
小鼠
91
65
8
克隆类型
单克隆
标记
This TNFRSF11A antibody is un-conjugated
9
7
7
6
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
应用范围
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
102
62
38
36
27
14
13
7
5
5
5
4
2
1
Supplier
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产品编号 ABIN252472
产品在该地区无法提供。
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.90991 ABIN252036 FACS IHC (p) IHC WB PE Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 10
13.90991 ABIN2663245 FACS PE Rat IgG2a kappa Log in to see R12-31 7
13.90991 ABIN2661015 FACS Biotin Rat IgG2a kappa Log in to see R12-31 4
13.90991 ABIN4349304 CyTOF FACS IP WB Mouse IgG Mix lambda Log in to see 64C1385-1 0
13.90991 ABIN4349337 FACS PE Mouse IgG Mix lambda Log in to see 64C1385-1 0
13.90991 ABIN4349336 CyTOF FACS IP WB Mouse IgG Mix lambda Log in to see 64C1385-1 0
1 ABIN2470154 FACS IHC WB Mouse IgG1 AA 326-616 Log in to see 9A725 0
1 ABIN4349315 FACS IHC IHC (p) WB DyLight 405 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 0
1 ABIN4349307 FACS IHC IHC (p) WB DyLight 650 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 0
1 ABIN4349331 FACS IHC IHC (p) WB Alexa Fluor 647 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 0
1 ABIN4349333 FACS IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 0
1 ABIN4349311 FACS IHC IHC (p) WB DyLight 755 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 0
1 ABIN4349312 FACS IHC IHC (p) WB PerCP Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 0
1 ABIN4349308 FACS IHC IHC (p) WB DyLight 680 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 0
1 ABIN4349327 FACS IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 0
1 ABIN4349305 FACS APC Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 0
1 ABIN4995452 FACS IHC IHC (p) FITC Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 0
1 ABIN2663246 FACS PE Rat IgG2a kappa Log in to see R12-31 7
1 ABIN119495 EIA FACS IP Rat IgG2a Log in to see LOB14-8 0
1 ABIN4349301 CyTOF FACS IHC IHC (p) WB Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 0

General

抗原 Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) 抗体
抗原表位 AA 326-616, AA 330-427
(20), (20), (16), (15), (10), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠, 大鼠
(143), (76), (51)
宿主 小鼠
(91), (65), (8)
克隆类型 (克隆位点)
单克隆   ( )
标记 This TNFRSF11A antibody is un-conjugated
(9), (7), (7), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
应用范围 Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(102), (62), (38), (36), (27), (14), (13), (7), (5), (5), (5), (4), (2), (1)
Pubmed 15个引用可参考
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TNFRSF11A抗体详情

TNFRSF11A目标详情 使用细节 贮存及处理 References for anti-TNFRSF11A antibody (ABIN252472) 图像
特异性 In RAW cells, a 97 kDa is observed.
纯化方法 Protein G purified
免疫原 A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830).
克隆位点 9A725
亚型 IgG1 kappa
Plasmids, Primers & others

TNFRSF11A目标详情

TNFRSF11A抗体详情 使用细节 贮存及处理 References for anti-TNFRSF11A antibody (ABIN252472) 图像 回页首
抗原
别名 RANK/TNFRSF11A (TNFRSF11A Antibody 摘要)
背景 Gene Symbol: TNFRSF11A
基因ID 8792
UniProt Q9Y6Q6
途径 NF-kappaB Signaling

使用细节

TNFRSF11A抗体详情 TNFRSF11A目标详情 贮存及处理 References for anti-TNFRSF11A antibody (ABIN252472) 图像 回页首
应用备注 Western Blot 1-2 μg/mL, Flow Cytometry 2-4 μg/mL, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Flow (Cell Surface), CyTOF-readyBharti et al (2004) and Reigel et al (2012) both published the RANK antibody clone 9A725 for Flow (Cell Surface). However, the epitope range recognized by this antibody is a potential cytoplasmic region as defined by www.uniprot.org. Hence, researchers are encouraged to refer to both the publication and the bioinformatics data bases for additional information in order to make their own determination regarding the suitability of the antibody for cell surface flow cytometry. Use in Flow cell surface reported in scientific literature (PMID 24376119)
说明

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

限制 仅限研究用

贮存及处理

TNFRSF11A抗体详情 TNFRSF11A目标详情 使用细节 References for anti-TNFRSF11A antibody (ABIN252472) 图像 回页首
状态 Liquid
浓度 1.0 mg/mL
缓冲液 PBS
Buffer contains: 0.05 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid freeze-thaw cycles
储存条件 4 °C,-20 °C
储存方法 Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-TNFRSF11A antibody (ABIN252472)

TNFRSF11A抗体详情 TNFRSF11A目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Watari, Shibata, Nabeshima, Shinoda, Fukunaga, Kawahara, Karasuyama, Fukushi, Iwamoto, Kuwano, Ono: "Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice." in: Scientific reports, Vol. 6, pp. 19470, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Nanke, Kobashigawa, Yago, Kawamoto, Yamanaka, Kotake: "RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients." in: BioMed research international, Vol. 2016, pp. 4874195, 2016 Method employed by authors: Western Blotting (WB)

Huynh, VonMoss, Smith, Rahman, Felemban, Zuo, Rody, McHugh, Holliday: "Characterization of Regulatory Extracellular Vesicles from Osteoclasts." in: Journal of dental research, Vol. 95, Issue 6, pp. 673-9, 2016 Method employed by authors: Western Blotting (WB)

Kaneko, Ota, Nakashima, Nagasaki, Kodani, Mori, Nagatsu: "Lipopolysaccharide treatment arrests the cell cycle of BV-2 microglial cells in G₁ phase and protects them from UV light-induced apoptosis." in: Journal of neural transmission (Vienna, Austria : 1996), Vol. 122, Issue 2, pp. 187-99, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Das, Sepahi, Duthie, Clark, Crockett: "RANK receptor oligomerisation in the regulation of NFκB signalling." in: Journal of molecular endocrinology, Vol. 53, Issue 1, pp. 81-91, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Lafferty, Fantry, Bryant, Jones, Hammoud, Weitzmann, Lewis, Garzino-Demo, Reid: "Elevated suppressor of cytokine signaling-1 (SOCS-1): a mechanism for dysregulated osteoclastogenesis in HIV transgenic rats." in: Pathogens and disease, Vol. 71, Issue 1, pp. 81-9, 2014 Method employed by authors: Flow Cytometry (FACS) (Sample species: Rat (Rattus)).

Riegel, Maurer, Prior, Stegmaier, Heppert, Wagner, Hänsch: "Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL." in: European journal of immunology, Vol. 42, Issue 4, pp. 975-81, 2012

Izawa, Zou, Chappel, Ashley, Feng, Teitelbaum: "c-Src links a RANK/αvβ3 integrin complex to the osteoclast cytoskeleton." in: Molecular and cellular biology, Vol. 32, Issue 14, pp. 2943-53, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting

Cannon, Kraj, Sloan: "Follicle-stimulating hormone promotes RANK expression on human monocytes." in: Cytokine, Vol. 53, Issue 2, pp. 141-4, 2011

Ayon Haro, Ukai, Yokoyama, Kishimoto, Yoshinaga, Hara: "Locally administered interferon-γ accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulation." in: Journal of periodontal research, Vol. 46, Issue 3, pp. 361-73, 2011

Crockett, Mellis, Shennan, Duthie, Greenhorn, Wilkinson, Ralston, Helfrich, Rogers: "Signal peptide mutations in RANK prevent downstream activation of NF-κB." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 26, Issue 8, pp. 1926-38, 2011 Further details: Western Blotting

Huang, Wen, Gao, Zhang, Bai: "An efficient and rapid method for cDNA cloning from difficult templates using codon optimization and SOE-PCR: with human RANK and TIMP2 gene as examples." in: Biotechnology letters, Vol. 33, Issue 10, pp. 1939-47, 2011 Further details: Western Blotting

Lee, Kim, Jun, Yoo, Woo, Choi, Lee, Song, Sohn, Park-Min, Ivashkiv, Ji: "Direct inhibition of human RANK+ osteoclast precursors identifies a homeostatic function of IL-1beta." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 10, pp. 5926-34, 2010 (Sample species: Human). Further details: Western Blotting

Bharti, Takada, Shishodia, Aggarwal: "Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 6065-76, 2004

Fiumara, Snell, Li, Mukhopadhyay, Younes, Gillenwater, Cabanillas, Aggarwal, Younes: "Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines." in: Blood, Vol. 98, Issue 9, pp. 2784-90, 2001

图像

TNFRSF11A抗体详情 TNFRSF11A目标详情 使用细节 贮存及处理 References for anti-TNFRSF11A antibody (ABIN252472) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) (AA 326-616), (AA 330-427) antibody (ABIN252472) Western Blot: RANK Antibody (9A725) [ABIN252472] - Western blot analysis of RANK in 2...
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) (AA 326-616), (AA 330-427) antibody (ABIN252472) Flow Cytometry: RANK Antibody (9A725) [ABIN252472] - Flow analysis of RANK in RAW cel...
 image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) (AA 326-616), (AA 330-427) antibody (ABIN252472) Flow (Cell Surface): RANK/TNFRSF11A Antibody (9A725) [ABIN2524728] - Analysis using t...
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) (AA 326-616), (AA 330-427) antibody (ABIN252472) Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - Using the PE direct conjugate An i...