anti-人 p65 抗体 for Electrophoretic Mobility-Shift Assay

Recommended p65 Antibody (supplied by: Log in to see )

抗原
Nuclear Factor-KB P65 (NFkBP65) 抗体
  • C-Rel
  • NFKB3
  • p65
  • NFkB
  • c-rel
  • zgc:100833
  • REL proto-oncogene, NF-kB subunit
  • RELA proto-oncogene, NF-kB subunit
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel avian reticuloendotheliosis viral oncogene homolog
  • REL
  • RELA
  • Rela
  • Rel
  • rel
抗原表位
N-Term, NLS Domain
131
85
69
63
49
45
41
38
38
37
26
26
25
18
17
16
15
15
13
13
13
12
11
10
8
8
7
6
6
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用

1151
668
520
72
56
55
30
25
25
22
13
8
8
6
4
2
1
1
宿主

1008
132
17
10
1
克隆类型
多克隆
标记
This p65 antibody is un-conjugated
30
29
23
20
18
18
17
13
12
12
12
8
8
8
8
8
8
8
8
4
4
3
3
2
2
2
2
2
2
1
应用范围
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
853
445
331
258
205
162
105
92
88
28
18
18
15
12
12
6
6
6
5
4
4
2
2
2
1
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产品编号 ABIN5556664
$ 636.30
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5556626 EMSA EIA IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN121827 EMSA EIA IP WB Sheep AA 12-317 Log in to see Polyclonal 0
1 ABIN121887 EMSA EIA IHC (fro) IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN117925 EMSA EIA IP WB Rabbit N-Term Log in to see Polyclonal 0

General

抗原 Nuclear Factor-KB P65 (NFkBP65) 抗体
抗原表位 N-Term, NLS Domain
(131), (85), (69), (63), (49), (45), (41), (38), (38), (37), (26), (26), (25), (18), (17), (16), (15), (15), (13), (13), (13), (12), (11), (10), (8), (8), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用
(1151), (668), (520), (72), (56), (55), (30), (25), (25), (22), (13), (8), (8), (6), (4), (2), (1), (1)
宿主
(1008), (132), (17), (10), (1)
克隆类型
多克隆
标记 This p65 antibody is un-conjugated
(30), (29), (23), (20), (18), (18), (17), (13), (12), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1)
应用范围 Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(853), (445), (331), (258), (205), (162), (105), (92), (88), (28), (18), (18), (15), (12), (12), (6), (6), (6), (5), (4), (4), (2), (2), (2), (1)
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p65抗体详情

p65目标详情 使用细节 贮存及处理 图像
序列 Peptide Sequence: R-I-E-E-K-R-K-R-T-Y-E-T
特异性 This affinity purified antibody is directed against the nuclear localization sequence (NLS) NLS of human p65 and is useful in determining its presence in various assays. The epitope recognized overlaps the NLS of the p65 subunit of the NFκB heterodimer. Therefore, the antibody selectively binds to the activated form of NFκB. Anti-NFκB p65 NLS may react non-specifically with other proteins.
产品特性 Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)
纯化方法 Affinity Purified
免疫原 This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkappaB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

p65目标详情

p65抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 RELA / NF-kB p65 (NFkBP65 Antibody 摘要)
背景 NFκB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFκB bound to IκB. NFκB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFκB subunit p65, similar to p50/p65 heterodimers. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IκB-α. IκB-α binds to the p65 subunit, preventing nuclear localization and DNA binding. IκB- α binding masks the NFκB nuclear localisation signal (NLS). A broad range of external stimuli lead to activation of NFκB and set off signalling cascades that ultimately converge on the IκB kinase (IKK) complex. Activated IKK specifically and directly phosphorylates IκB-α and this phophorylation event targets IκB-α for degradation. As a consequence, NFκB NLS is uncovered and nuclear translocation occurs.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65
基因ID 5970
NCBI登录号 NP_001138610
UniProt Q04206
途径 NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

使用细节

p65抗体详情 p65目标详情 贮存及处理 图像 回页首
应用备注 Suitable for Immunohistochemistry, Immunoblotting, ELISA and supershift assays. Thisproduct was assayed by Immunoblot and found to be reactive against NFκB p65 NLS at adilution of 1: 2,000 followed by reaction with Peroxidase conjugated Affinity Purified
限制 仅限研究用

贮存及处理

p65抗体详情 p65目标详情 使用细节 图像 回页首
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
储存条件 -20 °C
储存方法 Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.