anti-猪 Insulin 抗体 for Dot Blot

Recommended Insulin Antibody (supplied by: Log in to see )

抗原
Insulin (INS) 抗体
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
适用

950
468
369
350
239
194
45
11
9
8
7
7
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
宿主
豚鼠
784
196
48
9
4
2
1
克隆类型
多克隆
标记
This Insulin antibody is un-conjugated
52
41
36
21
20
20
20
15
15
15
13
13
13
13
13
13
13
10
9
9
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
应用范围
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Immunodiffusion (ID), Immunofluorescence (IF), Western Blotting (WB)
459
357
275
267
257
236
181
117
106
67
66
31
24
23
21
8
7
5
4
4
4
3
2
2
1
1
1
1
1
1
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产品编号 ABIN5540065
$ 828.00
加上运输费 $45.00

General

抗原 Insulin (INS) 抗体
适用
(950), (468), (369), (350), (239), (194), (45), (11), (9), (8), (7), (7), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
宿主 豚鼠
(784), (196), (48), (9), (4), (2), (1)
克隆类型
多克隆
标记 This Insulin antibody is un-conjugated
(52), (41), (36), (21), (20), (20), (20), (15), (15), (15), (13), (13), (13), (13), (13), (13), (13), (10), (9), (9), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
应用范围 Dot Blot (DB), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Immunodiffusion (ID), Immunofluorescence (IF), Western Blotting (WB)
(459), (357), (275), (267), (257), (236), (181), (117), (106), (67), (66), (31), (24), (23), (21), (8), (7), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Insulin抗体详情

Insulin目标详情 使用细节 贮存及处理 图像
特异性 The reactivity of the antiserum is directed to Insulin as tested by ELISA and Immunoblotting. No reaction was obtained with other Swine serum proteins.
纯化方法 Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt Precipitation and purification of the IgG fraction by DEAE Chromatography
免疫原 Insulin hormone is isolated from Porcine pancreas. Freund's complete adjuvant is used in the first step of the immunization procedure.
亚型 IgG
Plasmids, Primers & others

Insulin目标详情

Insulin抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 insulin (INS Antibody 摘要)
背景 Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds.
UniProt P01315
途径 NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

使用细节

Insulin抗体详情 Insulin目标详情 贮存及处理 图像 回页首
应用备注 The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates in radioimmunoassay for the production of immunoconjugates with a selected marker to prepare immunoaffinity adsorbents by coupling to an artificial carrier in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent in Western blotting. This product is not pre-diluted. Recommended Working Dilutions : Histochemical and Cytochemical Use: are usually between 1/100-1/250. In ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
限制 仅限研究用

贮存及处理

Insulin抗体详情 Insulin目标详情 使用细节 图像 回页首
缓冲液 PBS, pH 7.2 Preservatives: None, as it may interfere with the antibody activity. No foreign proteins added
储存液 Azide free
储存条件 4 °C,-20 °C
储存方法 Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
有效期 12 months