anti-人 Insulin 抗体 for Immunohistochemistry (Frozen Sections)

Recommended Insulin Antibody (supplied by: Log in to see )

抗原
Insulin (INS) 抗体
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
适用
人, 猪
988
495
381
374
235
189
47
10
8
7
6
6
5
2
2
2
2
1
1
1
1
1
1
1
1
1
宿主
小鼠
818
195
50
16
4
1
1
克隆类型
单克隆
标记
This Insulin antibody is un-conjugated
53
43
38
28
27
22
22
19
17
17
15
14
14
14
14
14
14
14
9
9
9
8
8
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
500
390
279
277
252
235
188
119
106
66
66
31
25
23
21
8
7
6
5
4
4
3
2
1
1
1
1
1
1
1
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产品编号 ABIN5556681
$ 619.30
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1804387 ELISA IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see E2E3 0
1 ABIN768892 ELISA IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see D3E7 0
1 ABIN1804388 ELISA IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see D3E7 (5B6-6) 0
1 ABIN112214 EIA IHC (fro) IHC (p) Mouse IgG1 Log in to see D3E7 (5B6-6) 1
1 ABIN255726 IHC (fro) IHC (p) IHC ELISA Mouse IgG1 Log in to see D3E7 (5B6-6) 0
1 ABIN487416 EIA IHC (fro) WB Mouse IgG1 Log in to see HPI-B5 0
1 ABIN4326102 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN111474 EIA IHC (fro) IHC (p) Mouse IgG1 Log in to see C-PEP-01 1
1 ABIN1723784 IHC (fro) IHC (p) ELISA Mouse IgG1 Log in to see D3E7 0
1 ABIN111464 EIA IHC (fro) IHC (p) Mouse IgG1 Log in to see C-PEP-01 2
1 ABIN295077 ELISA IHC IHC (fro) Mouse IgG1 C-Term Log in to see D4B8 0
1 ABIN111475 EIA IHC (fro) IHC (p) RIA Mouse IgG1 Log in to see C-PEP-01 1
1 ABIN4326030 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN115757 IHC (fro) IF IHC (p) Guinea Pig Log in to see Polyclonal 4
1 ABIN346524 ELISA IHC IHC (fro) Biotin Mouse kappa, IgG1 Log in to see D3E7 0
1 ABIN1839720 ELISA IHC IHC (fro) Mouse IgG1, kappa Log in to see D4B8 0
1 ABIN295205 ELISA IHC IHC (fro) Mouse IgG1 Log in to see D6C4 0
1 ABIN201081 ELISA IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see 0
1 ABIN345104 ELISA IHC IHC (fro) Mouse IgG1 Log in to see 0
1 ABIN112204 EIA IHC (fro) Mouse IgG1 Log in to see 5E4-3 0

General

抗原 Insulin (INS) 抗体
适用 人, 猪
(988), (495), (381), (374), (235), (189), (47), (10), (8), (7), (6), (6), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
宿主 小鼠
(818), (195), (50), (16), (4), (1), (1)
克隆类型 (克隆位点)
单克隆   ( )
标记 This Insulin antibody is un-conjugated
(53), (43), (38), (28), (27), (22), (22), (19), (17), (17), (15), (14), (14), (14), (14), (14), (14), (14), (9), (9), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
应用范围 Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(500), (390), (279), (277), (252), (235), (188), (119), (106), (66), (66), (31), (25), (23), (21), (8), (7), (6), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Insulin抗体详情

Insulin目标详情 使用细节 贮存及处理 图像
序列 Human Proinsulin (precursor) 21 aa A chain 90-110. 30 aa B chain 25-54. 1 MALWMRLLPL LALLALWGPD PAAAFVNQHL CGSHLVEALY LVCGERGFFY TPKTRREAED 61 LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN
特异性 This monoclonal 2D11.H5 antibody recognizes a polypeptide which is identified as Insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. This protein A purified Mouse monoclonal antibody reacts specifically with Insulin from Human and Swine sources. Cross reactivity with Insulin from Mouse and Rat does not occur. Cross reactivity with Insulin from other sources has not been determined.
Cellular Localization: Cytoplasmic.Cellular Localization:
产品特性 Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)
纯化方法 Protein A Chromatography
免疫原 Purified human Insulin coupled to Bovine Serum Albumin (BSA).
克隆位点 2D11-H5
亚型 IgG1
Plasmids, Primers & others

Insulin目标详情

Insulin抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 Insulin (INS Antibody 摘要)
背景 Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as β-cell and tumor (insulinoma) markers.Synonyms: INS
分子量 6 kDa
基因ID 3630
NCBI登录号 NP_000198
UniProt P01308
途径 NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

使用细节

Insulin抗体详情 Insulin目标详情 贮存及处理 图像 回页首
应用备注 This antibody is suitable for ELISA, Immunohistochemistry, Immunoblotting andimmunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or β-cellsfrom islets of Langerhans can be used as a positive control. Generally a 1: 50 to 1: 200 dilution is suitable for most applications. A 30 to 60 minuteincubation time is usually sufficient. However, depending upon the fixation conditions andthe staining system employed, optimal incubation should be determined by the user. Frozen sections of paraffin embedded tissues are recommended for preparations to use forimmunohistochemistry. No special pretreatment is required. Researchers shoulddetermine the optimal dilution for other specific assays and cell types. ELISA: Results of Mab anti-Insulin antibody were tested against human insulin by ELISA. Each well was coated with 0.1g of conjugate. The starting concentration of antibody in thedilution series was 10 μg/mL. Each point on the Y-axis represents a 3-fold dilution. Themidpoint of the titration curve represents approx-imately 5 ng/mL antibody or a 1: 200,000dilution from the stock concentration. HRP conjugated Gt-a- Mouse IgG H&L and TMBsubstrate were used for detection (see figure 1).
限制 仅限研究用

贮存及处理

Insulin抗体详情 Insulin目标详情 使用细节 图像 回页首
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
储存条件 -20 °C
储存方法 Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.