anti-Hamster Insulin 抗体 for Immunofluorescence

Recommended Insulin Antibody (supplied by: Log in to see )

抗原
Insulin (INS) 抗体
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
适用
Hamster, 人, 猪, 大鼠
949
468
369
350
238
194
45
11
9
8
7
7
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
宿主
豚鼠
784
196
48
9
4
2
1
克隆类型
多克隆
标记
This Insulin antibody is un-conjugated
52
41
36
21
20
20
20
15
15
15
13
13
13
13
13
13
13
10
9
9
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
459
356
275
267
257
237
180
118
106
67
66
31
25
23
21
8
7
5
4
4
4
3
2
2
2
1
1
1
1
1
1
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产品编号 ABIN115757
$ 830.50
加上运输费 $45.00

General

抗原 Insulin (INS) 抗体
适用 Hamster, 人, 猪, 大鼠
(949), (468), (369), (350), (238), (194), (45), (11), (9), (8), (7), (7), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
宿主 豚鼠
(784), (196), (48), (9), (4), (2), (1)
克隆类型
多克隆
标记 This Insulin antibody is un-conjugated
(52), (41), (36), (21), (20), (20), (20), (15), (15), (15), (13), (13), (13), (13), (13), (13), (13), (10), (9), (9), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
应用范围 Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(459), (356), (275), (267), (257), (237), (180), (118), (106), (67), (66), (31), (25), (23), (21), (8), (7), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4个引用可参考
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Insulin抗体详情

Insulin目标详情 使用细节 贮存及处理 References for anti-Insulin antibody (ABIN115757) 图像
特异性 The antibody recognizes Insulin produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors. Absorption with 10-100 μg proinsulin per ml diluted antiserum inactivates the antiserum, while C-peptide and Insulin only partly reduce staining.
交叉反应 (详细) Also reacts to: Proinsulin
纯化方法 Serum
免疫原 Synthetic Human proinsulin
Plasmids, Primers & others

Insulin目标详情

Insulin抗体详情 使用细节 贮存及处理 References for anti-Insulin antibody (ABIN115757) 图像 回页首
抗原
别名 Insulin (INS Antibody 摘要)
背景 Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS
基因ID 3630
NCBI登录号 NP_000198
UniProt P01308
途径 NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

使用细节

Insulin抗体详情 Insulin目标详情 贮存及处理 References for anti-Insulin antibody (ABIN115757) 图像 回页首
应用备注 Immunofluorescence: 1: 1000 - 1: 2000, overnight incubation at 2-8C. Immunohistochemistry on frozen and paraffin sections: 1: 3000 - 1: 5000. Positive Control: Formalin-fixed paraffin sections of human pancreas
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用

贮存及处理

Insulin抗体详情 Insulin目标详情 使用细节 References for anti-Insulin antibody (ABIN115757) 图像 回页首
状态 Liquid
溶解方式 Dissolve the antiserum in 50-100 μL distilled water, and dilute further in 0.1 M PBS with 1 % BSA and 0.09 % Sodium Azide
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody (in aliquots) at -20 °C.

References for anti-Insulin antibody (ABIN115757)

Insulin抗体详情 Insulin目标详情 使用细节 贮存及处理 图像 回页首
背景

Kohnert, Axcrona, Hehmke, Klöting, Sundler, Ahrén: "Islet neuronal abnormalities associated with impaired insulin secretion in type 2 diabetes in the Chinese hamster." in: Regulatory peptides, Vol. 82, Issue 1-3, pp. 71-9, 1999

Ahrén, Gudbjartsson, Al-Amin, Mårtensson, Myrsén-Axcrona, Karlsson, Mulder, Sundler: "Islet perturbations in rats fed a high-fat diet." in: Pancreas, Vol. 18, Issue 1, pp. 75-83, 1999

Myrsén-Axcrona, Karlsson, Sundler, Ahrén: "Dexamethasone induces neuropeptide Y (NPY) expression and impairs insulin release in the insulin-producing cell line RINm5F. Release of NPY and insulin through different pathways." in: The Journal of biological chemistry, Vol. 272, Issue 16, pp. 10790-6, 1997

Myrsén, Ahrén, Sundler: "Dexamethasone-induced neuropeptide Y expression in rat islet endocrine cells. Rapid reversibility and partial prevention by insulin." in: Diabetes, Vol. 45, Issue 10, pp. 1306-16, 1996