|抗原||Insulin (INS) 抗体|
|适用||雪貂, 人, 猪, 大鼠 其他选择|
|标记||This Insulin antibody is un-conjugated 其他选择|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|Supplier||Log in to see|
Insulin抗体详情Insulin目标详情 使用细节 贮存及处理 References for anti-Insulin antibody (ABIN113504) 图像
|特异性||The antibody detects Insulin which is produced by the B-cells of the pancreatic islets. Antibody BP5053 can be used for the diagnosis of pancreatic B-cell tumors. Antigen localization: cytoplasm, extracellular.|
Insulin目标详情Insulin抗体详情 使用细节 贮存及处理 References for anti-Insulin antibody (ABIN113504) 图像 回页首
|别名||Insulin (INS Antibody 摘要)|
|背景||Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS|
|途径||NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation|
使用细节Insulin抗体详情 Insulin目标详情 贮存及处理 References for anti-Insulin antibody (ABIN113504) 图像 回页首
ELISA. Immunohistochemistry on frozen and paraffin sections: proteolytic treatment is notrequired, optimal results have been obtained by using the autoclave or microwave retrievalmethod (with citrate buffer). For immunohistochemistry dilute 1: 50 - 1: 100 (with PBS or TBS immediately before use),based on the indirect method, with a normal ABC method the dilution can be up to 5xhigher when using the microwave retrieval method. Recommended positive control: Pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理Insulin抗体详情 Insulin目标详情 使用细节 References for anti-Insulin antibody (ABIN113504) 图像 回页首
|注意事项||Avoid repeated freezing and thawing.|
|储存条件||4 °C/-20 °C|
|储存方法||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Insulin antibody (ABIN113504)Insulin抗体详情 Insulin目标详情 使用细节 贮存及处理 图像 回页首
Luts, Sundler: "Peptide-containing nerve fibers in the respiratory tract of the ferret." in: Cell and tissue research, Vol. 258, Issue 2, pp. 259-67, 1990