anti-Yeast (Saccharomyces cerevisiae) Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 抗体 for Immunoprecipitation

Recommended Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 Antibody (supplied by: Log in to see )

抗原
Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8) 抗体
  • HIP6
  • HYPF
  • Nin1p
  • Rpn12
  • S14
  • p31
  • hip6
  • hypf
  • nin1p
  • rpn12
  • psmd8b
  • 6720456J22Rik
  • AA407360
  • AL033291
  • AL033322
  • AL033323
  • C76433
  • psmd8
  • psmd8a
  • fa93g07
  • fb17c09
  • fb49a10
  • zgc:86762
  • wu:fa93g07
  • wu:fb17c09
  • wu:fb49a10
  • PSMD8
  • proteasome 26S subunit, non-ATPase 8
  • proteasome 26S subunit, non-ATPase 8 S homeolog
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 8
  • proteasome 26S subunit, non-ATPase 8 L homeolog
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 pseudogene
  • PSMD8
  • psmd8.S
  • Psmd8
  • psmd8.L
  • psmd8
  • LOC468490
适用
Yeast (Saccharomyces cerevisiae)
47
32
27
1
1
1
1
1
宿主

37
8
2
克隆类型
多克隆
标记
非结合性
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunoprecipitation (IP), Western Blotting (WB)
29
16
13
8
7
4
2
2
2
1
选项
Supplier
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Supplier Product No.
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Available images

产品编号 ABIN2452112
$ 446.15
加上运输费 $45.00

General

抗原 Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8) 抗体
适用 Yeast (Saccharomyces cerevisiae)
(47), (32), (27), (1), (1), (1), (1), (1)
宿主
(37), (8), (2)
克隆类型
多克隆
标记 非结合性
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunoprecipitation (IP), Western Blotting (WB)
(29), (16), (13), (8), (7), (4), (2), (2), (2), (1)
Pubmed 3个引用可参考
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产品细节

目标详细情况 使用细节 贮存及处理 引用 图像
纯化方法 Affinity purified
免疫原 Recombinant yeast Rpn12 expressed in E. coli
亚型 IgG

目标详细情况

产品细节 使用细节 贮存及处理 引用 图像 回页首
抗原
别名 Rpn12 (PSMD8 Antibody 摘要)
背景 Background: The 26 S proteasome is a protein complex with a molecular mass of 2,000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation. The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. Rpn12 is one of the non-ATPase subunits of the lid. Rpn12 interacts with an ATPase subunit, Rpt1, of the base. Rpn12, Rpt1 double mutant becomes lethal, suggesting a strong interaction between Rpn12 and Rpt1. In the double mutant cells, the function of the 26S proteasome is completely eliminated.
途径 Mitotic G1-G1/S Phases, DNA Replication, Proton Transport, Synthesis of DNA

使用细节

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应用备注 1) Western blotting: 1/5,000~1/10,000
2) Immunoprecipitation
Other applications have not been tested.
限制 仅限研究用

贮存及处理

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状态 Liquid
缓冲液 PBS, 1 mg/mL BSA, 0.09 % sodium azide, 50 % glycerol
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C

引用

产品细节 目标详细情况 使用细节 贮存及处理 图像 回页首
有引用在:

Tone, Tanahashi, Tanaka, Fujimuro, Yokosawa, Toh-e: "Nob1p, a new essential protein, associates with the 26S proteasome of growing saccharomyces cerevisiae cells." in: Gene, Vol. 243, Issue 1-2, pp. 37-45, 2000

Takeuchi, Toh-e et al.: "Genetic evidence for interaction between components of the yeast 26S proteasome: combination of a mutation in RPN12 (a lid component gene) with mutations in RPT1 (an ATPase gene) causes synthetic ..." in: Molecular & general genetics : MGG, Vol. 262, Issue 1, pp. 145-53, 1999

Hershko, Ciechanover: "The ubiquitin system." in: Annual review of biochemistry, Vol. 67, pp. 425-79, 1998

图像

产品细节 目标详细情况 使用细节 贮存及处理 引用 回页首
厂商提供的图像
Western Blotting (WB) image for anti-Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8) antibody (ABIN2452112) anti-Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8) antibody
Western Blotting (WB) image for anti-Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8) antibody (ABIN2452112) anti-Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8) antibody (Image 2)