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抗Human DBF4 抗体:
抗Rat (Rattus) DBF4 抗体:
抗Mouse (Murine) DBF4 抗体:
Human Monoclonal DBF4 Primary Antibody for IF, ELISA - ABIN564742
Hughes, Jenkins, Dar, Engelman, Cherepanov: Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity. in The Journal of biological chemistry 2009
Mouse (Murine) Polyclonal DBF4 Primary Antibody for ELISA, WB - ABIN4304355
Tsuji, Lau, Chiang, Jiang: The role of Dbf4/Drf1-dependent kinase Cdc7 in DNA-damage checkpoint control. in Molecular cell 2008
CDC7-DBF4 kinase (DDK) has a primary role in the replication checkpoint to promote single-stranded DNA accumulation at stalled forks, which is required to initiate a robust checkpoint response and cell cycle arrest to maintain genome integrity.
Both CDC7 and DBF4 promoters bind E2F, suggesting that increased E2F activity in RB1 mutant cancers promotes increased DDK expression. Surprisingly, increased DDK expression levels are also correlated with both increased chemoresistance and genome-wide mutation frequencies.
Histone H3 lysine 9 (H3K9) acetylation was most prevalent when the Dbf4 transcription level was highest whereas the H3K9me3 level was greatest during and just after replication.
we propose that phosphorylation of TOP2A by CDC7/DBF4 in early S-phase prevents its localization and/or activity at centromeres, and inhibition of TOP2A function could be relevant to prevent premature separation of centromeric DNA.
The anti-invasive and cell cycle arrest-inducing effects of nitrogen-containing bisphosphonates are not DBF4 mediated in human breast cancer cells.
Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.
bipartite interaction between Cdc7 and Dbf4/ASK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase.
Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction
Data document the role of DBF4 as a key player in nitrogen-containing bisphosphonate-induced cytotoxicity, thus explaining the effects on the cell-cycle.
The interaction with LEDGF relieves autoinhibition of Cdc7-ASK kinase, imposed by the C terminus of ASK.
results suggest that E2F regulates the ASK promoter through an atypical mode of recognition of the target site
The HuDbf4 gene has of 12 exons over a 33-kb region. The MluI cell-cycle box is needed for core promoter activation. The Sp1 upregulator & HES-1 repressor coordinately determine promoter efficiency. Transcription initiations occur at -220, -235 and -245.
Results demonstrate a DNA damage checkpoint that targets Cdc7/Dbf4 protein kinase.
Cdc7-Dbf4 kinase efficiently phosphorylates p150.
Taken together, these results indicate that Cdc7/Dbf4 phosphorylation of MCM2 is essential for the initiation of DNA replication in mammalian cells.
Overexpression of Dbf4 abrogates the S checkpoint response to ultraviolet radiation but not ionizing radiation.
There is the differential regulation of a novel gene, namely ASK/Dbf4, in melanoma and suggest that up-regulation of ASK/Dbf4 is a novel molecular determinant with prognostic relevance that confers a proliferative advantage in cutaneous melanoma.
Cdc7/Dbf4 kinase activity inhibition affects specific phosphorylation sites on MCM2 in cancer cells
ASK/Dbf4, a novel cell survival gene in melanoma is transcriptionally regulated by E2F1.
A strong origin of replication at the DBF4 promoter locus, which contains two initiation zones, two origin recognition complex (ORC) binding sites and two DNase I-hypersensitive regions, is identified.
Regulatory subunit for cdc7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Not required during the initiation of DNA replication in egg and during early embryonic development but is required later and throughout development. The complex cdc7-dbf4a phosphorylates mcm2 subunit.
DBF4-type zinc finger-containing protein 1
, activator of S phase kinase
, chiffon homolog A
, protein DBF4 homolog A
, zinc finger, DBF-type containing 1
, DBF4 homolog (S. cerevisiae)
, protein DBF4 homolog A-like
, DBF4 homolog